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Immunology

The effects of Candida albicans and Candida krusei on expression of toll-like receptors and chemokine receptors by dendritic cells

Abstract

Resistance to candidiasis requires the coordinated action of innate and adaptive immunity. Dendritic cells (DCs) are the most crucial antigen presenting cells and playing important role in initiation of innate and adaptive immunity. The aim of this study is to investigate the effects of the different concentrations of C. albicans (Candida albicans) and C. krusei on expression of TLR2, TLR4, TLR6, CCR5, CXCR4 and CX3CR1 in in-vitro dendritic cell (DC) cultures.


There were twelve experimental models for each C. albicans and C. krusei strains. The six of them contained monocyte derived dendritic cells with a concentration of 2x106 cells/ml, six of them did not. While two control models did not contain yeast cells, the others contained yeast cells with the concentrations of 1x106 cells/ml, 2x106 cells/ml, 4x106 cells/ml, 8x106 cells/ml 10x106 cells/ml, respectively. Two sets of these culture plates were incubated at 35°C with 5% CO2, one set for 24 hours and one set for 48 hours. At the end of the incubation periods, TLR2, TLR4, TLR6, CCR5, CXCR4 and CX3CR1 expression levels were analyzed with ELISA.


There were distinct increases in TLR2, TLR4, TLR6, CCR5 and CXCR4 expression levels as C. albicans strain concentration increases in experiment models which included DCs. On the other hand, there were apparent increases in TLR2, TLR4, CCR5 and CXCR4 expression levels as C. krusei strain concentration increases in experiment models which include DCs. But there was no explicit difference between TLRs and chemokine receptor expression levels and C. albicans and C. krusei strain concentrations in experiment models without DCs.


The activation of DCs leads to the release of several inflammatory mediators such as TLRs and chemokines. TLRs and chemokines take a role in early phase of infection, TLRs take part in recognition and producing a signal, and chemokines take part a role in transmitting this signal to the effector cells.

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How to Cite This

Yesilyurt Sölen, E. . (2022). The effects of Candida albicans and Candida krusei on expression of toll-like receptors and chemokine receptors by dendritic cells. Jurnal Teknologi Laboratorium, 11(2). https://doi.org/10.29238/teknolabjournal.v11i2.353

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