Jurnal Teknologi Laboratorium https://teknolabjournal.com/index.php/Jtl <p style="text-align: justify;"><em>Jurnal Teknologi Laboratorium</em>, with registered number ISSN 2<a href="http://issn.pdii.lipi.go.id/issn.cgi?daftar&amp;1369726769&amp;1&amp;&amp;" target="_blank" rel="noopener">338-5634&nbsp;(print)</a>, <a href="http://issn.pdii.lipi.go.id/issn.cgi?daftar&amp;1493892253&amp;1&amp;&amp;" target="_blank" rel="noopener">2580-0191&nbsp;(online)</a>&nbsp;is a scientific journal published by&nbsp;Poltekkes Kemenkes Yogyakarta. The journal <span class="st">registered in the CrossRef system </span><span class="il">with </span><strong><span class="st">Digital Object Identifier </span><span class="il">(DOI)</span> prefix <a href="https://search.crossref.org/?q=2580-0191&amp;publication=Jurnal+Teknologi+Laboratorium" target="_blank" rel="noopener">10.29238.</a>&nbsp;</strong>&nbsp;The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have been achieved in the area of medical laboratory sciences.&nbsp; Every article that goes to the editorial staff will be selected through <strong>Initial Review</strong> processes by Editorial Board. Then, the articles will be sent to the Mitra Bebestari/ peer reviewer and will go to the next selection by Double-Blind<strong> Preview Process</strong>. After that, the articles will be returned to the authors to revise. These processes take a month for a maximum time. In each manuscript, Mitra Bebestari/ peer reviewer will be rated from the substantial and technical aspects. The final decision of articles acceptance will be made by Editors according to Reviewers comments.&nbsp;Mitra Bebestari/ peer reviewer that collaboration with <em>Jurnal Teknologi Laboratorium</em>&nbsp;is the experts in the medical laboratory technology&nbsp;area and issues around it.</p> <p style="text-align: justify;"><em>Jurnal Teknologi Laboratorium</em> particularly focuses on the main problems in the development of the sciences of medical laboratory&nbsp;areas. It covers the parasitology, bacteriology, virology, haematology, clinical chemistry, toxicology, food and drink chemistry.</p> <p style="text-align: justify;">In order to further internationalize our journal, Jurnal Teknologi Laboratorium has decided to change the Indonesian language to<strong> accept with English or Indonesian</strong> starting from volume 7 issue 1 March 2018. Due to this change, the article guideline of Jurnal Teknologi Laboratorium will be renewed accordingly.</p> <p style="text-align: justify;"><strong>Please read these guidelines carefully</strong>. &nbsp;Authors who want to submit their&nbsp;manuscript to the editorial office of&nbsp;<em>Jurnal Teknologi Laboratorium</em>&nbsp;should obey the writing guidelines. If the&nbsp;manuscript submitted is not appropriate with the guidelines or written in a different format, <strong>it will&nbsp;BE REJECTED</strong> by the editors before further reviewed. The editors will only accept the manuscripts which meet the assigned format.</p> <table style="background-color: #ccffff;" width="100%"> <tbody> <tr> <td style="width: 100%; text-align: center;"> <h2>Statistics</h2> </td> </tr> <tr> <td style="width: 581px;"> <p style="padding-left: 30px;"><strong>Volume 7 No 1 2018:</strong></p> <p style="padding-left: 30px;">Articles Received: 13; Accepted: 8; Rejected: 5; Published: 5; Retracted: 0</p> </td> </tr> </tbody> </table> en-US <p style="text-align: justify;">Publishing your paper with Jurnal Teknologi Laboratorium&nbsp;(JTL)&nbsp;means that the author or authors retain the copyright in the paper. JTL&nbsp;granted an exclusive reuse license by the author(s), but the author(s) are able to put the paper onto a website, distribute it to colleagues, give it to students, use it in your thesis etc, even commercially. The author(s) can reuse the figures and tables and other information contained in their paper published by JTL&nbsp;in future papers or work without having to ask anyone for permission, provided that the figures, tables or other information that is included in the new paper or work properly references the published paper as the source of the figures, tables or other information, and the new paper or work is not direct at private monetary gain or commercial advantage.</p> <p style="text-align: justify;">JTL&nbsp;journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. This journal is licensed under&nbsp;a&nbsp;<a href="http://creativecommons.org/licenses/by-sa/4.0/" rel="license">Creative Commons Attribution-ShareAlike 4.0 International License</a>.&nbsp;This license lets others remix, transform, and build upon the material&nbsp;for any purpose, even commercially.</p> <p style="text-align: justify;">JTL&nbsp;journal Open Access articles are distributed under this <strong><a href="http://creativecommons.org/licenses/by-sa/4.0/" rel="license">Creative Commons Attribution-ShareAlike 4.0 International License</a>&nbsp;(CC BY-SA).</strong>&nbsp;Articles can be read and shared for All&nbsp;purposes under the following conditions:</p> <ul> <li class="show" style="text-align: justify;"><em>BY:</em>&nbsp;You must give <a id="appropriate_credit_popup" class="helpLink" href="https://creativecommons.org/licenses/by-sa/4.0/#">appropriate credit</a>, provide a link to the license, and&nbsp;<a id="indicate_changes_popup" class="helpLink" href="https://creativecommons.org/licenses/by-sa/4.0/#">indicate if changes were made</a>. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.</li> <li class="show" style="text-align: justify;"><em>SA:</em>&nbsp; If you remix, transform, or build upon the material, you must distribute your contributions under the <a id="same_license_popup" class="helpLink" href="https://creativecommons.org/licenses/by-sa/4.0/#">same license</a> as the original.</li> </ul> teknolabjournal@gmail.com (Budi Setiawan) teknolabjournal@gmail.com (Budi Setiawan) Thu, 03 May 2018 00:00:00 +0000 OJS http://blogs.law.harvard.edu/tech/rss 60 Isolation of Candida albicans from Swab Mucosa Mouth Patient Diabetes Mellitus Type 2 https://teknolabjournal.com/index.php/Jtl/article/view/103 <p>Candida albicans can be pathogen when immunity had decreased and physiological function is impaired, such as in diabetes mellitus type 2. This study aims to isolation Candida albicans that collected from the oral cavity of diabetes mellitus type 2 patients. This research is conducted with a descriptive study by observing the presence of Candida albicans in 30 samples of diabetes mellitus type 2 patients, which grows on Potato Dextrose Agar. The microscopic observation by LPCB staining of yeasts, blastospores, pseudohyphae, chlamydospores and germ tubes in human serum suspension that incubated at 370C for 2-3 hours. Based on this research was found 14 (46,7%) patients from 30 patients were positive Candida albicans.</p> Ni Kadek Sri Jayanti, I Nyoman Jirna ##submission.copyrightStatement## http://creativecommons.org/licenses/by-sa/4.0 https://teknolabjournal.com/index.php/Jtl/article/view/103 Thu, 03 May 2018 00:00:00 +0000 Immunomodulator of Ethanol Extracts of The Leaves Azadirachta indica Against Macrophage Peritoneal Cell in Mice Induced The Vaccine BCG https://teknolabjournal.com/index.php/Jtl/article/view/110 <p><em>This research was conducted to determine the immunomodulatory effect of ethanol extract of neem leaves (Azadirachta indica) to the number of peritoneal macrophages in mice wich induced by BCG vaccine. Bacillus Calmette-Guerin (BCG) vaccine contained an attenuated Mycobacterium bovis. Mycobacterium bovis belongs to the Mycobacterium Tuberculosis Complex (MTC) group that has a similar phenotype characteristic with Mycobacterium tuberculosis and similar clinical manifestations of tuberculosis.The type of the research that used in this study is laboratory experimental research with Post Test Design Design Only Control Group Design. The research was conducted at the Faculty of Veterinary Medicine of Airlangga University in July 2017 using 25 male mice divided into five groups. The dosage of ethanol extract of the neem leaves given was 200 mg / Kg BW with variation for two days, four days, and six days are given.In the result of statistical data analysis using Kruskal-walis test, it is known that the significance value p = 0,03 (p &lt;0,05), that means immunomodulatory of ethanol extract of neem leaves (Azadirachta indica) give an effect to peritoneal macrophage cell number in mice wich induced by BCG vaccine, so that neem leaves ethanol extract can be applied to tuberculosis patients.</em></p> Yogi Khoirul Abror, Evy Diah Woelansari, Suhariyadi Suhariyadi ##submission.copyrightStatement## http://creativecommons.org/licenses/by-sa/4.0 https://teknolabjournal.com/index.php/Jtl/article/view/110 Thu, 03 May 2018 00:00:00 +0000 The Optimization of Secondary Metabolite Production Time and Screening Antibacterial Activity of Actinomycetes Isolate from Tin Plant Rizosfer (Ficus carica) https://teknolabjournal.com/index.php/Jtl/article/view/120 <p style="text-align: justify;">Some Actinomycetes isolates of a tin plant (<em>Ficus carica</em> L.) have been obtained, namely T24M, T18, T19, T24, T25, T34, T37, T41, and T43. The aim of this study was to optimize the production of secondary metabolites (antibiotics) and screening antibacterial activity against Methicillin-Resistant Staphylococcus aureus (MRSA) from the Actinomycetes isolate of the tin rhizosphere. The study was performed with test an activity of the culture fluid from Actinomycetes isolate against MRSA by the well method. The result of optimization secondary metabolite production showed that the second day was the best incubation time to harvest antibiotics. The results showed that bacterial isolates of T24M produced antibiotics that could inhibit MRSA growth.</p> Warsi Warsi, Nanik Sulistyani ##submission.copyrightStatement## http://creativecommons.org/licenses/by-sa/4.0 https://teknolabjournal.com/index.php/Jtl/article/view/120 Thu, 03 May 2018 00:00:00 +0000 Potention of Antibacterial Isolat Actinomycetes to Proteolitic and Amilolitic Activity Escherichia Coli ATTC 25922 https://teknolabjournal.com/index.php/Jtl/article/view/101 <p style="text-align: justify;">Occurs E. coli resistance to class 3 cephalosporin class antibiotics and fluoroquinolone groups. The antibiotic resistance that occurs has narrowed the choice of therapy. This study aims to determine the effect of Actinomycetes isolates on proteolytic and amylolytic enzymes <em>E. coli</em> ATCC 25922. This research is experimental research, qualitative tests of protease and amylase enzymes from <em>E. coli</em> ATCC 25922 shown by clear zones around the growing colonies. The result of <em>ANOVA One-Way</em> test showed a significant difference in the width of clear zone, colony zone and <em>PER and AER</em> score with p-value &lt; 0,05. This indicates that Actinomycetes isolates contain compounds that can act as inhibitors of protease and amylase enzymes from <em>E.coli </em>ATCC 25922. It is hoped that there will be research about the identification of Actinomycetes species isolates in Bogor Botanical Garden so that later can be cultivated and produced as an antibiotics alternative.</p> Meiskha Bahar, Fajriati Zulfa ##submission.copyrightStatement## http://creativecommons.org/licenses/by-sa/4.0 https://teknolabjournal.com/index.php/Jtl/article/view/101 Thu, 03 May 2018 00:00:00 +0000 The Variation of Alpha Cyclodextrin and Centrifugation Time in Lipemic Serum Preparation for Glucose Examination With GOD-PAP Method https://teknolabjournal.com/index.php/Jtl/article/view/121 <p>Examination of glucose levels of the GOD-PAP method in serum may be disrupted by the presence of turbidity caused by lipemic serum, thus causing high false serum glucose levels to result. The addition of alpha-cyclodextrin (α-CD) may bind the lipemic in serum. This study aims to find out how the concentration and time of optimal centrifugation with the addition of alpha-cyclodextrin. Added variation alpha-cyclodextrin concentration 0.5%, 1%, and 1.5% in pooled serum with variation concentration of triglycerides ± 1000 mg/dL, ± 1500 mg/dL and ± 2000 mg/dL incubated for 30 minute at 4°C. Centrifuged at 3000 rpm with variation of centrifugation time for 5 minutes, 10 minutes and 15 minutes at 4°C to form precipitate and supernatant. Then the supernatant measured the glucose GOD-PAP and triglycerides GPO-PAP using a photometer. The result were no significant differences in variations centrifugation time and variations alpha-cyclodextrin concentrations after comparison with serum glucose pooled levels (base line) in the ANOVA test, Kruskall Wallis Test and Mann Whitney test. There was no significant difference with pooled serum (base line) at the time of triglyceride concentration ± 1000 mg/dL with alpha-cyclodextrin concentration 0.5% and centrifugation time at 10 minute, triglyceride concentration ± 1500 mg/dL and ± 2000 mg/dL with alpha-cyclodextrin concentration 1%, centrifugation time at 5 minute.</p> Arfa Izzati, Ani Riyani ##submission.copyrightStatement## http://creativecommons.org/licenses/by-sa/4.0 https://teknolabjournal.com/index.php/Jtl/article/view/121 Thu, 03 May 2018 00:00:00 +0000