Abstract
The emergence of multi-drug resistant (MDR) bacteria has endangered the efficacy of antibiotics treatment of pathogenic bacteria worldwide. The aim of this research was to investigate the incidence of Salmonella enterica serovar Typhi in Duhok city, Iraq. Specimens of blood and stool were recruited from 267 patients. S. Typhi isolates were diagnosed depending on morphology, biochemical and serological tests. S. Typhi isolates were tested for their antibiotic resistance. Multi-drug resistant S. Typhi isolates were conjugated with E. coli HB101. The plasmid profile of transconjugants was investigated. 15/267 (5.6%) S. Typhi isolates were identified. Based on their biochemical tests, S. Typhi isolates were categorized into two biotypes (I, 26.66% and II, 73.33%). Four resistance patterns were observed. The resistant pattern to ampicillin and tetracycline was the higher (46.6%). Conjugation experiment showed that all antibiotic markers were transferred from S. Typhi to E. coli HB101 with a conjugation frequency of (0.38×10-5). 13.3% of the S. Typhi isolates were multi-drug-resistant resistant and had two small plasmids. Transconjugants E. coli acquired the resistance from the multi-drug resistant S. Typhi. Antibiotics treatment of the pathogens could be hindered by the constant rise of multi-drug-resistant. Further studies are needed to study the mobile genetic elements and their contribution to antibiotics resistance.
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References
2. Mastroeni P and Menager N, Development of acquired immunity to Salmonella. J Med Microbiol.2003;52:453-9.
3. Habeeb A, Hussein NR, Assafi MS, and Al-Dabbagh SA, Methicillin resistant Staphylococcus aureus nasal colonization among secondary school students at Duhok City-Iraq. J Microbiol Infect Dis.2014;4:59-63.
4. Assafi MS, Mohammed RQ, and Hussein NR, Nasal carriage rates of Staphylococcus aureus and ca-methicillin resistant Staphylococcus aureus among university students. Int. J. Microbiol. Res.2015;5:123-7.
5. Yousefi-Mashouf R and Moshtaghi A, Frequency of typhoidal and non-typhoidal salmonella species and detection of their drugs resistance patterns. J Res Health Sci.2007;7:49-56.
6. Thomas CM and Nielsen KM, Mechanisms of, and barriers to, horizontal gene transfer between bacteria. Nat Rev Microbiol.2005;3:711-21.
7. Mazel D, Dychinco B, Webb VA, and Davies J, Antibiotic resistance in the ECOR collection: integrons and identification of a novel aad gene. Antimicrob Agents Chemother.2000;44:1568-74.
8. Hall MM, Ilstrup DM, and Washington JA, 2nd, Comparison of three blood culture media with tryptic soy broth. J Clin Microbiol.1978;8:299-301.
9. Prescott LM, Harley JP, and Klein DA, Microbiology. 5th ed. 2002, New York: McGraw–Hill Companies.
10. Gillespie SH and Hawkey PM, Principles and Practice of Clinical Bacteriology 2nd ed. 2006, Chichester: John Wiley & Sons.
11. Bauer AW, Kirby WM, Sherris JC, and Turck M, Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol.1966;45.
12. CLSI, Clinical and Laboratory Standards Institutes (CLSI). Performance Standards for Antimicrobial Susceptibility Testing; Seventeenth Informational Supplement. M100-S17. 2007;27.
13. Buu-Hoi A and Horodniceanu T, Conjugative transfer of multiple antibiotic resistance markers in Streptococcus pneumoniae. J Bacteriol.1980;143:313-20.
14. Raeder V and Broda P, Rapid preparation of DNA from filamentous fungi L. Appl. Microbiol.1985;1:17-20.
15. Edelstein M, Pimkin M, Dmitrachenko T, et al., Multiple outbreaks of nosocomial salmonellosis in Russia and Belarus caused by a single clone of Salmonella enterica serovar Typhimurium producing an extended-spectrum beta-lactamase. Antimicrob Agents Chemother.2004;48:2808-15.
16. Ochiai RL, Acosta CJ, Danovaro-Holliday MC, et al., A study of typhoid fever in five Asian countries: disease burden and implications for controls. Bull World Health Organ.2008;86:260-8.
17. Basnyat B, Maskey AP, Zimmerman MD, and Murdoch DR, Enteric (typhoid) fever in travelers. Clin Infect Dis.2005;41:1467-72.
18. Sen B, Dutta S, Sur D, et al., Phage typing, biotyping & antimicrobial resistance profile of Salmonella enterica serotype Typhi from Kolkata. Indian J Med Res.2007;125:685-8.
19. Iseri L, Bayraktar MR, Aktas E, and Durmaz R, Investigation of an outbreak of Salmonella typhi in Battalgazi district, Malatya-Turkey. Braz J Microbiol.2009;40:170-3.
20. Cooke FJ and Wain J, The emergence of antibiotic resistance in typhoid fever. Travel Med Infect Dis.2004;2:67-74.
21. Misra RN, Bawa KS, Magu SK, Bhandari S, Nagendra A, and Menon PK, Outbreak of Multi-drug Resistant Salmonella typhi Enteric Fever in Mumbai Garrison. Med J Armed Forces India.2005;61:148-50.
22. Baliga S, Drug Resistance in Salmonella typhi: Tip of the Iceberg. Online J Health Allied Scs 2004;4.
23. Alanis AJ, Resistance to antibiotics: are we in the post-antibiotic era?, Arch Med Res , 2005, vol. 36 (pg. 697-705). Arch Med Res.2005;36:697-705.
24. Assafi MS, Polse RF, Hussein NR, Haji AH, and Issa AR, The Prevalence of S. aureus Nasal Colonisation and its Antibiotic Sensitivity Pattern amongst Primary School Pupils. Science Journal of University of Zakho.2017;5:7-10.
25. Hussein NR, Assafi MS, and Ijaz T, Methicillin-resistant Staphylococcus aureus nasal colonisation amongst healthcare workers in Kurdistan Region, Iraq. J Glob Antimicrob Resist.2017;9:78-81.
26. Hussein NR, Muhammed AH, Al-Dabbagh S, Abdulkareem WL, and Assafi MS, The Prevalence of USA300 CA-MRSA in Kurdistan Region, Northern Iraq. Int. J. Pure Appl. Sci. Technol.2014;21:8-10.
27. Mills-Robertson F, Addy ME, Mensah P, and Crupper SS, Molecular characterization of antibiotic resistance in clinical Salmonella typhi isolated in Ghana. FEMS Microbiol Lett.2002;215:249-53.
28. Waldvogel FA and Pitton JS, Typhoid fever imported from Mexico to Switzerland. Studies on R factor mediated chloramphenicol resistance. J Hyg (Lond).1973;71:509-13.
29. Mandal S, Mandal MD, and Pal NK, R-Factor in Salmonella enterica serovar typhi: transfer to and acquisition from Escherichia coli. Jpn J Infect Dis.2003;56:65-7.
30. Mandal S, Mandal MD, and Pal NK, Plasmid-Encoded Multidrug Resistance of Salmonella typhi and some Enteric Bacteria in and around Kolkata, India: A Preliminary Study. J Health Allied Scs.2004;3:1-7.
31. Rychlik I, Gregorova D, and Hradecka H, Distribution and function of plasmids in Salmonella enterica. Vet Microbiol.2006;112:1-10.
32. Mandal S, Mandal MD, and Pal NK, Combination effect of ciprofloxacin and gentamicin against clinical isolates of Salmonella enterica serovar typhi with reduced susceptibility to ciprofloxacin. Jpn J Infect Dis.2003;56:156-7.
33. Lawley TD, Gilmour MW, Gunton JE, Standeven LJ, and Taylor DE, Functional and mutational analysis of conjugative transfer region 1 (Tra1) from the IncHI1 plasmid R27. J Bacteriol.2002;184:2173-80.
34. Wain J, Diem Nga LT, Kidgell C, et al., Molecular analysis of incHI1 antimicrobial resistance plasmids from Salmonella serovar Typhi strains associated with typhoid fever. Antimicrob Agents Chemother.2003;47:2732-9.
35. Bennett PM, Plasmid encoded antibiotic resistance: acquisition and transfer of antibiotic resistance genes in bacteria. British Journal of Pharmacology.2008;153:S347–S357.
36. Chen W, Fang T, Zhou X, Zhang D, Shi X, and Shi C, IncHI2 Plasmids Are Predominant in Antibiotic-Resistant Salmonella Isolates. Front Microbiol.2016;7:1566.
37. Carattoli A, Plasmid-mediated antimicrobial resistance in Salmonella enterica. Curr Issues Mol Biol.2003;5:113-22.
38. Pasquali F, Kehrenberg C, Manfreda G, and Schwarz S, Physical linkage of Tn3 and part of Tn1721 in a tetracycline and ampicillin resistance plasmid from Salmonella Typhimurium. J Antimicrob Chemother.2005;55:562-5.
39. Krauland MG, Marsh JW, Paterson DL, and Harrison LH, Integron-mediated multidrug resistance in a global collection of nontyphoidal Salmonella enterica isolates. Emerg Infect Dis.2009;15:388-96.
40. Al-Sanouri TM, Paglietti B, Haddadin A, et al., Emergence of plasmid-mediated multidrug resistance in epidemic and non-epidemic strains of Salmonella enterica serotype Typhi from Jordan. J Infect Dev Ctries.2008;2:295-301.
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