Antibacterial Activity of Karika Dieng Peels Againts Shigella flexneri and Escherichia coli

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Dhiah Novalina Sugiyarto Sugiyarto Ari Susilowati

Abstract

Karika is an endemic plant of Dieng Plateau. The leaf of Karika has been studied to have antibacterial activity against bacteria that cause diarrhea, while the peels are removed or used as a mixture of animal feed. This study aims to determine the antibacterial activity of Karika peels against bacteria that cause poisoning, i.e Shigella flexneri, and Escherichia coli. The sample fractionated to obtain the fraction of n-hexane and ethyl acetate. The fractions were tested for their antibacterial activity against Shigella flexneri and Escherichia coli by the cup-plate method. The results showed that the ethyl acetate fraction (ethyl acetate 50%) had the highest inhibition on Shigella flexneri and Escherichia coli compared with another concentration. Based on the study can be concluded that the peels of Karikahas antibacterial activity against Shigella flexneri and Escherichia coli.

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How to Cite
Novalina, D., Sugiyarto, S., & Susilowati, A. (2018). Antibacterial Activity of Karika Dieng Peels Againts Shigella flexneri and Escherichia coli. Jurnal Teknologi Laboratorium, 7(2), 53-60. https://doi.org/https://doi.org/10.29238/teknolabjournal.v7i2.137
Section
Bacteriology

References

1. Pracoyo N. E., dan Parwati D.Analisis Mikrobiologik Beberapa Jenis Makanan Jajanan (Moko) di DKI Jakarta. Jakarta: Cermin Dunia Kedokteran; 2006.
2. Siagian A. Mikroba Patogen pada Makanan dan Sumber Pencemarannya. Medan: USU Digital Library; 2002.
3. Coleman ME, Marks HM, Golden NJ, Latimer HK. Discerning Strain Effects in Microbial Dose-Response Data. Journal of Toxicology and Environmental Health. 2004; 67: 667-85
4. Lesmana M.Enterobacteriaceae: Salmonella&Shigella. Jakarta: Fakultas Kedokteran Universitas Trisakti; 2003.
5. Dewi SK. Analisis Strategi Pengembangan Usaha Industri Kecil Olahan Carica. Departemen Agribisnis Fakultas Ekonomi dan Manajemen. Institut Pertanian Bogor: Bogor; 2009.
6. Hidayat S. Prospek Pepaya Gunung (Carica pubescens Lenne & K. Koch) dari Sikunang, Pegunungan Dieng, Wonosobo. UPT Balai Pengembangan Kebun Raya LIPI: Bogor; 2000.
7. Muhamad SAS, Jamilah B., Russly AR and Faridah A. In vitro antibacterial activities and composition of Carica papaya cv. Sekaki/ HongKong Peel Extracts. International Food Research Journal. 2017; 24(3): 976-984.
8. Nirosha N and Mangalanayaki R. Antibacterial Activity of Leaves and Stem Extract of Carica papaya L. IJAPBC. 2013; 2(3).
9. Peter JK, Kumar Y, Pandey P and Masih H. Antibacterial Activity of Seed and Leaf Extract of Carica papaya var Pusa Dwarf Linn. Journal of Pharmacy and Biological Sciences. 2014; 9(2): 29-37.
10. Wilda Y, Satryani FM, Maemonah S, Badriyah, Christyaningsih J. The Inhibitory Effect of Carica papaya cv. Thailand Leaf Extract to the Growth of Enterococcus faecalis In Vitro. Scholars Journal of Dental Sciences. 2017; 4(6):262-266 .
11. Novalina Dhiah, Sugiyarto, Susilowati A. Aktivitas Antibakteri Ekstrak DaunCarica Pubescens Dari Dataran Tinggi Dieng Terhadap Bakteri Penyebab Diare. El-Vivo. 2013; 1(1): 1-12.
12. Alfiah I. Aktivitas Antibakteri Fraksi Etil Asetat Ekstrak Etanol Daun Karika Terhadap Salmonella typhi secara in Silico dan in vitro. Malang: Jurusan biologi Universitas islam negeri maulana malik ibrahim malang; 2016.
13. Putri GI dan Nurhayani. Uji Aktivitas Antibaktri Ekstrak Etanol 70% Kulit Buah Carica (Carica pubescens) Terhadap Bakteri Salmonella typhi. Universitas Muhammadiyah Surakarta: Surakarta; 2018.
14. Puspawati N. Aktivitas Antimikrobia dan Profil Kromatografi Lapis Tipis dari Ekstrak Meniran Merah (Phyllanthus urinaria) dan Hasil Fraksinasinya terhadap Staphylococcus aureus ATCC 25923.Program Pascasarjana Universitas Sebelas Maret: Surakarta; 2011.
15. Ahmed D, Waheed A, Chaudhary MA, Khan SR, Hannan A and Barkaat M. Nutritional and Antimicrobial Studies on Leaves and Fruit of Carissa opaca Stapf Ex Haines. EJEAF Che,. 2010; 9 (10): 1631-1640.
16. Kumala S, Shanny F dan Wahyudi P. Aktivitas Aantimikroba Metabolit Bioaktif Mikroba Endofitik Tanaman Trengguli (Cassia fistula L.). Jurnal Farmasi Indonesia. 2006; 3(2): 97-102.
17. Abeysinghe PD. Antibacterial Activity of some Medicinal Mangroves against Antibiotic Resistant Pathogenic Bacteria. Indian J. Pharm. Sci. 2010;72 (2): 167-172.
18. Fitrial Y, Astawan M, Soekarto SS, Wiryawan KG, Wresdiyati T, Khairina R. Aktivitas Antibakteri Ekstrak Biji Teratai Terhadap Bakteri Patogen Penyebab Diare. J. Teknologi dan Industri Pangan. 2008;19(2).
19. Boutellaa S, Zellagui A, Baaziz N and Hallis Y.Assessment of Antimicrobial Activity of Ethyl Acetate and n-butanol Extracts from Satureja graeca L. growing in Algeria.Der Pharmacia Lettre. 2016; 8(19):462-465 .
20. Solomon S, Muruganantham N, Senthamilselvi MM. Antimicrobial Activity of Tabebuia rosea (bunga). International Journal of Research and Development in Pharmacy and Life Sciences. 2016; 5(2): 2018-2022.
21. Wetungu MW.,Matasyoh JC, Kinyanjui T.Antimicrobial activity of solvent extracts from the leaves of Tarchonanthus camphoratus (Asteraceae). Journal of Pharmacognosy and Phytochemistry. 2014; 3(1): 123-127.
22. Haque U, RahmanA, Haque A, Sarker AK, Islam AU. Antimicrobial and Anticancer Activities of Ethyl Acetate Extract of Co-culture of Streptomyces sp. ANAM-5 and AIAH-10 Isolated From Mangrove Forest of Sundarbans, Bangladesh. Journal of Applied Pharmaceutical Science. 2016; 6(02):051-055.
23. Kanazawa A, Ikeda T and Endo T. A novel Approach to Mode of Action of Cationic Biocides Morphological Effect on Bacterial Activity. J. Appl. Bacterial. 1995; 78: 55-60.
24. Lee Dae-Sung, Sung-Hwan E, Jae-Young J, Young-Mo K, Myung-Suk L and Young-Man K. Antibacterial Activity of an Ethyl Acetate Extract of Pseudomonas sp. UJ-6 against Methicillin-Resistant Staphylococcus aureus. Fish Aquat Sci. 2013;16 (2): 79-84.
25. Fitrial Y. Aktivitas Antibakteri Ekstrak Etil Asetat Biji Teratai (Nymphaea pubescens Willd) Akibat Pemanasan.Jurnal Pengolahan Hasil Perikanan Indonesia. 2011; 14(1): 43-48.