Potential of Lactobacillus casei shirota’s strain against the biofilm-forming of Salmonella Spp

: an in vitro study

Main Article Content

Annisa Rizka Fauziah Meiska Bahar Aprilla Ayu Wulandari


Biofilm of Salmonella spp. is formed through the expression of biofilm genes associated with proteins (bapA) regulated by curli synthesis genes (csg) which carry out adhesion, colonization, maturation, and dispersion on the surface of the intestinal epithelium. This study aimed to determine the antibiofilm activity of Lactobacillus casei Shirota’S strain (LcS) as an inhibitor of Salmonella spp. biofilm formation in vitro. This research was a true experimental study using Microtiter Plate 96 wells Biofilm Assay method. The sample used was the suspension of Salmonella spp. The treatment was in the form of adding a LcS suspension with a concentration series of 10-1;10-2; 10-3;10-4; and 10-5. Biofilm measurements were carried out using a microplate reader and obtained quantitative data in the form of Optical Density at a wavelength of 595nm. The results of this study showed that LcS suspension has antibiofilm activity ranging from 10-5 concentrations with a percentage of 36.58% (p<0.05). The results of exometabolism LcS can reduce Salmonella growth. Exopolysaccharide (EPS) and sortase-dependent proteins (SrtA) of LcS form barriers as competitive adhesion in inhibiting pathogenic biofilm formation.


Download data is not yet available.

Article Details

How to Cite
Fauziah, A., Bahar, M., & Ayu Wulandari, A. (2019). Potential of Lactobacillus casei shirota’s strain against the biofilm-forming of Salmonella Spp. Jurnal Teknologi Laboratorium, 8(2), 54 - 63. https://doi.org/https://doi.org/10.29238/teknolabjournal.v8i2.165


1. World Health Organization (WHO). Estimates of the global burden of foodborne diseases: foodborne disease burden epidemiology reference group 2007-2015. Geneva: WHO; 2018.
2. Center for Disease Control and Prevention – CDC. National Center for Emerging and Zoonotic Infectious Diseases (NCEZID), Division of Foodborne, Waterborne, and Environmental Diseases (DFWED). USA; 2019.
3. Riset Kesehatan Dasar, Badan Litbang Kesehatan 2007, ‘Laporan Nasional Riskesdas 2007’. Jakarta; 2008
4. Peng, D. Biofilm Formation of Salmonella’. IntechOpen Microbial Biofilms - Importance and Applications. 2016; 241-231.
5. Simoes, M, Simoes, LC, Machado, I, Pereira, MO, Vieira, MJ. A review of current and emergent biofilm control strategies. LWT - Food Science and Technology. 2010; 43.
6. Yana, X, Gu, S, Cui, X, Shi, Y, Wen, S, Chen, H, Ge, J 2019. Antimicrobial, anti-adhesive and anti-biofilm potential of biosurfactants isolated from Pediococcus acidilactici and Lactobacillus plantarum against Staphylococcus aureus CMCC26003. Microbial Pathogenesis. 2019; 127: 12-20.
7. Biswas D, Mengfei, P. Linoleic Acids Overproducing Lactobacillus casei Limits Growth, Survival, and Virulence of Salmonella Typhimurium and Enterohaemorrhagic Escherichia coli. Frontiers in Microbiology. 2018; 9.
8. Gómez, NC, Juan, M, Ramiro, P, Beatriz, X, Quecan, V, Bernadette, D, de Melo Franco, G. Use of Potential Probiotic Lactic Acid Bacteria ( LAB ) Biofilms for the Control of Listeria monocytogenes, Salmonella Typhimurium, and Escherichia coli O157 : H7 Biofilms Formation. Front. Microbiol. 2016; 1–15.
9. Rahayu, WP 2001, Penuntun Praktikum Penilaian Organoleptik. Bogor: Fakultas Teknologi Pertanian, Institut Pertanian Bogor: Bogor; 2001
10. Ahmed, Z, Wang, Y, Cheng, Q, Imran, I 2010, ‘Lactobacillus acidophillus bacteriocin, from production to their application: an overview’. African Journal of Biotechnology. 2001; 9 (20).
11. Fujimoto, J, Matsuki, T, Sasamoto, M, Tomii, Y, Watanabe, K. Identification and quantification of Lactobacillus casei strain Shirota in human feces with strain-specific primers derived from randomly amplified polymorphic DNA. Int J Food Microbiol. 2008; 126:210–15.
12. O'Toole, GA. Microtiter dish biofilm formation assay. Journal of visualized experiments. 2011: JoVE;47.
13. Clinical and Laboratory Standards Institute 2012. Performance standards for antimicrobial susceptibility testing; twentieth informational supplement. Document M100-S22. Wayn., USA: 2012; 3(1).
14. Borges, S, Silva, J, Teixeira, P 2012. Survival and biofilm formation by Group B streptococci in simulated vaginal fluid at different pHs. A Van Leeuw. 2012: 101; 677–682.
15. Stevenson, K, McVey, AF, Clark, IB, Swain, PS, Pilizota, T. General calibration of microbial growth in microplate readers. Scientific Reports. 2016.
16. Koohestani, M, Moradi, M, Tajik, H, Badali, A 2018. Effects of cell-free supernatant of Lactobacillus acidophilus LA5 and Lactobacillus casei 431 against planktonic form and biofilm of Staphylococcus aureus. Veterinary Research Forum. 2018: 9(4); 301–306.
17. Salas-Jara, MJ, Ilabaca, A, Vega, M, García, A 2016. Biofilm Forming Lactobacillus: New Challenges for the Development of Probiotics. Microorganisms. 2016: 4(35)
18. Rumbaugh, KP 2014. Antibiofilm Agents. Springer Series on Biofilms Series. England; 2014: 8; 175-67.