Detection of B1 gene as Toxoplasmosis marker in women of childbearing age in West Bandung Regency, Indonesia

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Patricia Gita Naully Selvi Anggraeni Supendi

Abstract

Congenital toxoplasmosis can cause damage and death to the fetus, to prevent this case, toxoplasmosis testing is important for the woman of childbearing age. One of the methods to screening the presence of T. gondii in the blood is Polymerase Chain Reaction (PCR). One of the T. gondii genes which can be used as a marker is the B1 gene. There are many toxoplasmosis cases in Indonesia, but the data is still difficult to find in West Bandung Regency. This study aimed to determine the number of toxoplasmosis cases in a woman of childbearing age in West Bandung Regency using the B1 gene as a marker and to determine the factors that influence these cases by conducting statistical analysis on the results of the questionnaire. The sample used in this study was 50 women of childbearing age (got married and domiciled in West Bandung). All samples have met the inclusion criteria and signed the informed consent. DNA from blood specimens was isolated using the Wizard Genomic DNA Purification Kit. The concentration and purity of isolated DNA were measured using a nanodrop device. Besides, the B1 gene from T. gondii was amplified using a pair of specific primers and visualized by the agarose electrophoresis method. Data were analyzed using the logistic regression method. The results showed that 7 women of childbearing age women (14%) in West Bandung Regency had toxoplasmosis. Frequent contact with pets, especially cats, was a significant factor (p <0.005) in this disease transmission.

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How to Cite
Naully, P., & Supendi, S. (2020). Detection of B1 gene as Toxoplasmosis marker in women of childbearing age in West Bandung Regency, Indonesia. Jurnal Teknologi Laboratorium, 9(2), 168-175. https://doi.org/https://doi.org/10.29238/teknolabjournal.v9i2.204
Section
Parasitology
Author Biographies

Patricia Gita Naully, Department of Medical Laboratory Technology, School of Health Sciences, Jenderal Achmad Yani Cimahi, Indonesia

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Patricia Gita Naully, Completed her Bachelor's degree in Microbiology Study Program, School of Life Sciences and Technology, Bandung Institute of Technology in 2014.  She joined the fast track program in Biotechnology Study Program, School of Life Sciences and Technology, Bandung Institute of Technology and completed her master's degree in 2015.  Since 2016, she became a lecturer in Medical Laboratory Technology Study Program, Jenderal Achmad Yani College of Health Sciences. Subjects taught Cell Biology, Molecular Biology, Immunology, Virology, and Bioinformatics.  Actively researching in the fields of detection of sexually transmitted infections using serological and molecular methods, development of molecular methods for detecting pathogens, and development of hepatitis B vaccine.

Selvi Anggraeni Supendi, Department of Medical Laboratory Technology, School of Health Sciences, Jenderal Achmad Yani Cimahi, Indonesia

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Selvi Anggraeni Supendi, has been working as a laboratory medical technologist at Pondok Indah Hospital and actively conducting molecular-based examinations. She is a member of the Indonesian Medical Laboratory Technology Association.

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