Identification of Salmonella typhi contamination by amplification fliC gene in grass-jelly from traditional markets and minimarket in Semarang city

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Aditya Rahman Ernanto Junita Rensa Palimbongan Anjar Richardo Manufandu Sri Darmawati


Grass-jelly is one of the most popular plants consumed by people in various forms. Contamination can cause various diseases, one of those is typhoid fever by Salmonella typhi. The purpose of this study was to detect S. typhi in grass-jelly based on molecular detection by amplification of the fliC gene using PCR. Validation was done by culture methods on SSA media and biochemical testing. The fliC gene amplification results in grass-jelly samples (A1, A2, B1, B2, C1, and C3) showed the DNA fragments size of about 1500 bp. Colony and biochemical characters isolate Peterongan were lead to S. typhi, whereas another isolate was another Salmonella spp. Grass-jelly samples from the Peterongan market in Semarang were positively contaminated by S. typhi and isolate from Pedurungan and the minimarket was another Salmonella spp. Molecular-based food testing is fast enough and accurate for detecting types of bacterial contaminants but the amplification of only the fliC gene cannot specific for S. typhi.


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Ernanto, A., Palimbongan, J., Manufandu, A., & Darmawati, S. (2020). Identification of Salmonella typhi contamination by amplification fliC gene in grass-jelly from traditional markets and minimarket in Semarang city. Jurnal Teknologi Laboratorium, 9(2), 136-144.
Author Biographies

Aditya Rahman Ernanto, Department of Medical Laboratory Technology, Universitas Muhammadiyah Semarang, Semarang, Indonesia


Aditya Rahman ERNANTO. He graduated from Universitas Gadjah Mada, Faculty of Biology in 2017, focused study on Genetics and Molecular Biology. He worked as a lecturer in Universitas Muhammadiyah Semarang, Faculty of Nursing and Health Science since 2018 focus study on Molecular Biology and Microbiology.

Junita Rensa Palimbongan, Department of Medical Laboratory Technology, Faculty of Nursing and Health, Universitas Muhammadiyah Semarang, Indonesia


Junita Rensa PALIMBONGAN. She was born in Nabire, Papua in 1996. She graduated from D-IV Technology Laboratory Technology Faculty of Nursing and Health Science in 2019. She is working in The General Hospital of the Nabire, Papua district.

Anjar Richardo Manufandu, Department of Medical Laboratory Technology, Faculty of Nursing and Health, Universitas Muhammadiyah Semarang, Indonesia


Anjar Richardo MANUFANDU. He has been an undergraduate student in D-IV Technology Laboratory Technology Faculty of Nursing and Health Science since 2017. He works as an assistant laboratory in Molecular Biology Laboratory.

Sri Darmawati, Department of Medical Laboratory Technology, Faculty of Nursing and Health, Universitas Muhammadiyah Semarang, Indonesia


Sri DARMAWATI. She is an associate professor in the Medical Laboratory Science Magister Program Universitas Muhammadiyah Semarang, a lecturer, and researcher on Microbiology and Biomolecular field. She was passionate about research on the special genetic diversity of Salmonella typhi.


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