Anti-inflammatory activities of squalene compound of methanol extract of Abroma augusta L

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Madyawati Latief Muhaimin Muhaimin Hilda Amanda Graha Prahandika Indra Lasmana Tarigan

Abstract

Abroma augusta L plant traditionally was used to treat swellings, cuts, sores, and bruises. In the province of Jambi, A. augusta is used in folk medicine to treat wounds. This study aims to isolate the steroid compound from the root of A. augusta L and determine its anti-inflammatory activities. Extraction and fractionation have been done with graded maceration using solvents with different polarities, which are n-hexane, ethyl acetate, and methanol. The separation was performed by column chromatography, followed by preparative thin-layer chromatography. The characterization of the isolate was carried out using UV-Vis spectrophotometry and infrared spectrophotometry, GC-MS. The anti-inflammatory activities of methanol extract and isolate of A. augusta was performed in this study was designed to evaluate the dose-response relationship of the anti-inflammatory activity in rat models of chronic inflammation chromatography to obtain isolate 2.1.1 that characterize and showed maximum absorbance at 265. The result of IR showed the presence of functional groups, -C=C-H, -C=H, -CH, CH3, CH2, and –CO belongs to the steroid compound. The results of the GC-MS show that isolates contain squalene compounds with a value of m/z 410, Isolate and crude extract showed an anti-inflammatory activity that almost approached the positive control of sodium 4-chlorophenolate. It could be concluded that isolate and extract provide good anti-inflammatory activity, that promise for new drug candidate squalene-based A. augusta.

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How to Cite
Latief, M., Muhaimin, M., Amanda, H., Prahandika, G., & Tarigan, I. (2020). Anti-inflammatory activities of squalene compound of methanol extract of Abroma augusta L. Jurnal Teknologi Laboratorium, 9(2), 176-185. https://doi.org/https://doi.org/10.29238/teknolabjournal.v9i2.228
Section
Clinical Chemistry
Author Biographies

Madyawati Latief, Fakultas Sains dan Teknologi, Universitas Jambi, Indonesia

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Dr. Madyawati Latief, S.P., M.Si. was born in Jambi on June 24, 1972. Completing a Bachelor's Degree in the BDP-Soil Science Study Program, Jambi University in 1995. A master's degree was obtained from Andalas University in 1999 in the chemistry study program, field of Organic Chemistry. Continuing his doctoral studies at Padjajaran University in the chemistry study program, majoring in Organic Chemistry and graduating in 2008. Currently working as a lecturer at Jambi University since 1999 in several subjects, including organic chemistry, bioactivity, natural product, and structural elucidation of organic compounds. Her research focuses on the chemical field of natural ingredients as alternative medicinal compounds and has published several articles in both national and international journals.

Muhaimin Muhaimin, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi, Indonesia

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Prof. Dr. rer. nat. Muhaimin, S.Pd, M.Si was Born in Tanjung Batu, South Sumatra, on March 22, 1973. Completed his bachelor's degree in the Chemical Education Study Program, Department of PMIPA, Sriwijaya University in 1997. His master’s degree was completed in 2000 at the Bandung Institute of Technology, Department of Chemistry, concentration in Organic Chemistry. He conducts a Doctoral degree at the College of Pharmacy, Freie Universität Berlin - Germany, and graduated in 2013. On June 1, 2020, he was confirmed as a Professor in the field of Organic Chemistry. From 2000 until currently, he has been a lecturer at the Chemistry Education Study Program, FKIP Jambi University, and since 2020 he has appointed as Head of the Department and lecturer at the Department of Pharmacy, Faculty of Medicine and Health Sciences, Jambi University. Subjects taught Organic Chemistry, Physical Organic Chemistry, Synthetic Organic Chemistry, Structure Elucidation of Organic Compounds, Pharmaceutical Chemistry, Analytical Pharmaceutical Chemistry, Scientific Work, and Research Methods. His research interest in the fields of Organic Synthesis Chemistry, Organic Chemistry of Natural Materials, Pharmacognosy, Pharmacology, and Pharmaceutical Technology. There have been dozens of research articles published in reputable international journals or indexed in Scopus and the Web of Science.

Hilda Amanda, Fakultas Sains dan Teknologi, Universitas Jambi, Indonesia

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Hilda Amanda, S.Pd., M.Si. Born in Koto Baru Hiang, Kerinci, Jambi on May 1, 1991. Completed his Bachelor's Degree in Chemistry Education, Faculty of Mathematics and Natural Sciences, Jambi University in 2013. His master's education was completed in 2015 at Andalas University, Padang, majoring in Chemistry with a concentration in Organic Chemistry. Have a research interest in natural products and bioactivity.

Graha Prahandika, Fakultas Sains dan Teknologi, Universitas Jambi, Indonesia

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Graha Prahandika, S.Si was born in Maro Sebo, January 9, 1996. Is an alumnus of the Chemistry Study Program, Faculty of Science and Technology, Jambi University. Entered 2014 and completed his studies in 2018. Has a research interest in Organic Chemistry of Natural Materials, under the guidance of Dr. Madyawati Latief, S.P., M.Si and Prof. Dr.rer.nat. Muhaimin, S.Pd., M.Si.

Indra Lasmana Tarigan, Fakultas Sains dan Teknologi, Universitas Jambi, Indonesia

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Indra Lasmana Tarigan, S.Pd., M.Sc was born in Pancur Batu, North Sumatra, on May 28, 1992. He was completed a bachelor's degree in the Chemistry Education Study Program, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Medan State University in 2014. His master's education was completed in 2018 at National Central University, Taiwan with a Department of Life Sciences with a concentration in Biochemistry and Molecular Biology. Currently as a Lecturer at the Chemistry Study Program, Faculty of Science and Technology, Jambi University. His research interest in Biochemistry, Natural Product as Antibacterial, Anti-fungal, and Anti-inflammatory agents.

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