Identification of molecular markers for type 2 Diabetes mellitus in Sidoarjo, Indonesia
Abstract
T2DM can be triggered by two collaborating factors, namely genetics and the environment. This study aimed to identify genetic markers that can be used to detect the possibility of a person having T2D using the random amplified polymorphism DNA (RAPD) method. The study was carried out cross-sectional and involved 60 samples consisting of 30 positive T2D samples and 30 negative samples T2D. The primer used for PCR-RAPD was D20 (5'-ACCCGGTCAC-3’). The PCR-RAPD results were then analyzed using the scoring method and analyzed using the non-parametric Chi-Square test (cl: 95%). Among T2D, 576 bp band were confirmed to be markers in the patients.
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References
2. Alves C, Casqueiro J, Casqueiro J. Infections in patients with diabetes mellitus: A review of pathogenesis. Indian J Endocrinol Metab. 2012;16(7):27. doi:10.4103/2230-8210.94253.
3. Pititto B de A, Ferreira SRG. Diabetes and covid-19: more than the sum of two morbidities. Rev Saude Publica. 2020;54:54. doi:10.11606/s1518-8787.2020054002577.
4. International Diabetes federation-IDF. Diabetes Atlas. 7th ed. Belgium: International Diabetes Federation; 2015. https://diabetesatlas.org/en/resources/.
5. Lyssenko V, Laakso M. Genetic Screening for the Risk of Type 2 Diabetes: Worthless or valuable? Diabetes Care. 2013;36(Supplement_2):S120-S126. doi:10.2337/dcS13-2009.
6. Karalliedde J, Gnudi L. Diabetes mellitus, a complex and heterogeneous disease, and the role of insulin resistance as a determinant of diabetic kidney disease. Nephrol Dial Transplant. 2014;31(2):gfu405. doi:10.1093/ndt/gfu405.
7. Mushlih M. Difference of Red Blood Cell Count (RBC) Levels in Diabetes Mellitus Type II with Ulcers and without Ulcers. J Ris Biol dan Apl. 2020;2(1):6-10.
8. Biadgo B, Melku M, Mekonnen Abebe S, Abebe M. Hematological indices and their correlation with fasting blood glucose level and anthropometric measurements in type 2 diabetes mellitus patients in Gondar, Northwest Ethiopia. Diabetes, Metab Syndr Obes Targets Ther. 2016;9:91. doi:10.2147/DMSO.S97563.
9. Hu C, Jia W. Diabetes in China: Epidemiology and Genetic Risk Factors and Their Clinical Utility in Personalized Medication. Diabetes. 2018;67(1):3-11. doi:10.2337/dbi17-0013.
10. Yu J. Genetics in Diabetes Mellitus - Contribution to the Classification and Management. Ann Pediatr Endocrinol Metab. 2012;17(4):211. doi:10.6065/apem.2012.17.4.211.
11. Andersson DK, Petersson C. Screening for Type 2 Diabetes. Diabetes Care. 2004;27(Supplement 1):S11-S14. doi:10.2337/diacare.27.2007.S11.
12. Chen J, Meng Y, Zhou J, et al. Identifying Candidate Genes for Type 2 Diabetes Mellitus and Obesity through Gene Expression Profiling in Multiple Tissues or Cells. J Diabetes Res. 2013;2013:1-9. doi:10.1155/2013/970435.
13. Ingelsson E, McCarthy MI. Human Genetics of Obesity and Type 2 Diabetes Mellitus. Circ Genomic Precis Med. 2018;11(6):2090. doi:10.1161/CIRCGEN.118.002090.
14. Tsaih S-W, Holl K, Jia S, et al. Identification of a Novel Gene for Diabetic Traits in Rats, Mice, and Humans. Genetics. 2014;198(1):17-29. doi:10.1534/genetics.114.162982.
15. van Tilburg J. Defining the genetic contribution of type 2 diabetes mellitus. J Med Genet. 2001;38(9):569-578. doi:10.1136/jmg.38.9.569.
16. Leitner DR, Frühbeck G, Yumuk V, et al. Obesity and Type 2 Diabetes: Two Diseases with a Need for Combined Treatment Strategies - EASO Can Lead the Way. Obes Facts. 2017;10(5):483-492. doi:10.1159/000480525.
17. Spanakis EK, Golden SH. Race/Ethnic Difference in Diabetes and Diabetic Complications. Curr Diab Rep. 2013;13(6):814-823. doi:10.1007/s11892-013-0421-9.
18. Asif M. The prevention and control the type-2 diabetes by changing lifestyle and dietary pattern. J Educ Health Promot. 2014;3(1):1. doi:10.4103/2277-9531.127541.
19. Zahid RA, Sulaiman BK, Abd A, B. Molecular Investigation of Genetic Polymorphisms in Type 2 Diabetic Patients Using Random Amplified Polymorphic DNA (RAPD-PCR). Iraqi J Cancer Med Genet. 2011;4(2):47–54. https://www.iasj.net/iasj/article/56172.
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