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Clinical Chemistry

Identification of molecular markers for type 2 Diabetes mellitus in Sidoarjo, Indonesia

Miftahul Mushlih Department Medical Laboratory Technology, Universitas Muhammadiyah Sidoarjo, Sidoarjo, Jawa Timur, Indonesia, Indonesia Correspondence mif.mushlih@umsida.ac.id
,
Fitri Kumala Sari Department Medical Laboratory Technology, Universitas Muhammadiyah Sidoarjo, Sidoarjo, Jawa Timur, Indonesia, Indonesia Correspondence khumalasarifitri@gmail.com
,
Hindah Sabrina Amin Department Medical Laboratory Technology, Universitas Muhammadiyah Sidoarjo, Sidoarjo, Jawa Timur, Indonesia, Indonesia Correspondence hindahsabrina@gmail.com
,
Siti Asriani Iknan Department Medical Laboratory Technology, Universitas Muhammadiyah Sidoarjo, Sidoarjo, Jawa Timur, Indonesia, Indonesia Correspondence asrianimoudy12@gmail.com
Pages 186-191
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Abstract

T2DM can be triggered by two collaborating factors, namely genetics and the environment. This study aimed to identify genetic markers that can be used to detect the possibility of a person having T2D using the random amplified polymorphism DNA (RAPD) method. The study was carried out cross-sectional and involved 60 samples consisting of 30 positive T2D samples and 30 negative samples T2D. The primer used for PCR-RAPD was D20 (5'-ACCCGGTCAC-3’). The PCR-RAPD results were then analyzed using the scoring method and analyzed using the non-parametric Chi-Square test (cl: 95%). Among T2D, 576 bp band were confirmed to be markers in the patients.

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How to Cite This

Mushlih, M., Sari, F. K., Amin, H. S., & Iknan, S. A. (2020). Identification of molecular markers for type 2 Diabetes mellitus in Sidoarjo, Indonesia. Jurnal Teknologi Laboratorium, 9(2), 186–191. https://doi.org/10.29238/teknolabjournal.v9i2.235

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