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Clinical Chemistry

Identification of molecular markers for type 2 Diabetes mellitus in Sidoarjo, Indonesia

Miftahul Mushlih Department Medical Laboratory Technology, Universitas Muhammadiyah Sidoarjo, Sidoarjo, Jawa Timur, Indonesia, Indonesia Correspondence mif.mushlih@umsida.ac.id
,
Fitri Kumala Sari Department Medical Laboratory Technology, Universitas Muhammadiyah Sidoarjo, Sidoarjo, Jawa Timur, Indonesia, Indonesia Correspondence khumalasarifitri@gmail.com
,
Hindah Sabrina Amin Department Medical Laboratory Technology, Universitas Muhammadiyah Sidoarjo, Sidoarjo, Jawa Timur, Indonesia, Indonesia Correspondence hindahsabrina@gmail.com
,
Siti Asriani Iknan Department Medical Laboratory Technology, Universitas Muhammadiyah Sidoarjo, Sidoarjo, Jawa Timur, Indonesia, Indonesia Correspondence asrianimoudy12@gmail.com
Pages 186-191
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Abstract

T2DM can be triggered by two collaborating factors, namely genetics and the environment. This study aimed to identify genetic markers that can be used to detect the possibility of a person having T2D using the random amplified polymorphism DNA (RAPD) method. The study was carried out cross-sectional and involved 60 samples consisting of 30 positive T2D samples and 30 negative samples T2D. The primer used for PCR-RAPD was D20 (5'-ACCCGGTCAC-3’). The PCR-RAPD results were then analyzed using the scoring method and analyzed using the non-parametric Chi-Square test (cl: 95%). Among T2D, 576 bp band were confirmed to be markers in the patients.

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References

1. Saeedi P, Petersohn I, Salpea P, et al. Global and regional diabetes prevalence estimates for 2019 and projections for 2030 and 2045: Results from the International Diabetes Federation Diabetes Atlas, 9th edition. Diabetes Res Clin Pract. 2019;157:107843. doi:10.1016/j.diabres.2019.107843.
2. Alves C, Casqueiro J, Casqueiro J. Infections in patients with diabetes mellitus: A review of pathogenesis. Indian J Endocrinol Metab. 2012;16(7):27. doi:10.4103/2230-8210.94253.
3. Pititto B de A, Ferreira SRG. Diabetes and covid-19: more than the sum of two morbidities. Rev Saude Publica. 2020;54:54. doi:10.11606/s1518-8787.2020054002577.
4. International Diabetes federation-IDF. Diabetes Atlas. 7th ed. Belgium: International Diabetes Federation; 2015. https://diabetesatlas.org/en/resources/.
5. Lyssenko V, Laakso M. Genetic Screening for the Risk of Type 2 Diabetes: Worthless or valuable? Diabetes Care. 2013;36(Supplement_2):S120-S126. doi:10.2337/dcS13-2009.
6. Karalliedde J, Gnudi L. Diabetes mellitus, a complex and heterogeneous disease, and the role of insulin resistance as a determinant of diabetic kidney disease. Nephrol Dial Transplant. 2014;31(2):gfu405. doi:10.1093/ndt/gfu405.
7. Mushlih M. Difference of Red Blood Cell Count (RBC) Levels in Diabetes Mellitus Type II with Ulcers and without Ulcers. J Ris Biol dan Apl. 2020;2(1):6-10.
8. Biadgo B, Melku M, Mekonnen Abebe S, Abebe M. Hematological indices and their correlation with fasting blood glucose level and anthropometric measurements in type 2 diabetes mellitus patients in Gondar, Northwest Ethiopia. Diabetes, Metab Syndr Obes Targets Ther. 2016;9:91. doi:10.2147/DMSO.S97563.
9. Hu C, Jia W. Diabetes in China: Epidemiology and Genetic Risk Factors and Their Clinical Utility in Personalized Medication. Diabetes. 2018;67(1):3-11. doi:10.2337/dbi17-0013.
10. Yu J. Genetics in Diabetes Mellitus - Contribution to the Classification and Management. Ann Pediatr Endocrinol Metab. 2012;17(4):211. doi:10.6065/apem.2012.17.4.211.
11. Andersson DK, Petersson C. Screening for Type 2 Diabetes. Diabetes Care. 2004;27(Supplement 1):S11-S14. doi:10.2337/diacare.27.2007.S11.
12. Chen J, Meng Y, Zhou J, et al. Identifying Candidate Genes for Type 2 Diabetes Mellitus and Obesity through Gene Expression Profiling in Multiple Tissues or Cells. J Diabetes Res. 2013;2013:1-9. doi:10.1155/2013/970435.
13. Ingelsson E, McCarthy MI. Human Genetics of Obesity and Type 2 Diabetes Mellitus. Circ Genomic Precis Med. 2018;11(6):2090. doi:10.1161/CIRCGEN.118.002090.
14. Tsaih S-W, Holl K, Jia S, et al. Identification of a Novel Gene for Diabetic Traits in Rats, Mice, and Humans. Genetics. 2014;198(1):17-29. doi:10.1534/genetics.114.162982.
15. van Tilburg J. Defining the genetic contribution of type 2 diabetes mellitus. J Med Genet. 2001;38(9):569-578. doi:10.1136/jmg.38.9.569.
16. Leitner DR, Frühbeck G, Yumuk V, et al. Obesity and Type 2 Diabetes: Two Diseases with a Need for Combined Treatment Strategies - EASO Can Lead the Way. Obes Facts. 2017;10(5):483-492. doi:10.1159/000480525.
17. Spanakis EK, Golden SH. Race/Ethnic Difference in Diabetes and Diabetic Complications. Curr Diab Rep. 2013;13(6):814-823. doi:10.1007/s11892-013-0421-9.
18. Asif M. The prevention and control the type-2 diabetes by changing lifestyle and dietary pattern. J Educ Health Promot. 2014;3(1):1. doi:10.4103/2277-9531.127541.
19. Zahid RA, Sulaiman BK, Abd A, B. Molecular Investigation of Genetic Polymorphisms in Type 2 Diabetic Patients Using Random Amplified Polymorphic DNA (RAPD-PCR). Iraqi J Cancer Med Genet. 2011;4(2):47–54. https://www.iasj.net/iasj/article/56172.
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How to Cite This

Mushlih, M., Sari, F. K., Amin, H. S., & Iknan, S. A. (2020). Identification of molecular markers for type 2 Diabetes mellitus in Sidoarjo, Indonesia. Jurnal Teknologi Laboratorium, 9(2), 186–191. https://doi.org/10.29238/teknolabjournal.v9i2.235

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