Identification of molecular markers for type 2 Diabetes mellitus in Sidoarjo, Indonesia

Main Article Content

Miftahul Mushlih Fitri Kumala Sari Hindah Sabrina Amin Siti Asriani Iknan

Abstract

T2DM can be triggered by two collaborating factors, namely genetics and the environment. This study aimed to identify genetic markers that can be used to detect the possibility of a person having T2D using the random amplified polymorphism DNA (RAPD) method. The study was carried out cross-sectional and involved 60 samples consisting of 30 positive T2D samples and 30 negative samples T2D. The primer used for PCR-RAPD was D20 (5'-ACCCGGTCAC-3’). The PCR-RAPD results were then analyzed using the scoring method and analyzed using the non-parametric Chi-Square test (cl: 95%). Among T2D, 576 bp band were confirmed to be markers in the patients.

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How to Cite
Mushlih, M., Sari, F., Amin, H., & Iknan, S. (2020). Identification of molecular markers for type 2 Diabetes mellitus in Sidoarjo, Indonesia. Jurnal Teknologi Laboratorium, 9(2), 186-191. https://doi.org/https://doi.org/10.29238/teknolabjournal.v9i2.235
Section
Clinical Chemistry
Author Biographies

Miftahul Mushlih, Department Medical Laboratory Technology, Universitas Muhammadiyah Sidoarjo, Sidoarjo, Jawa Timur, Indonesia

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Miftahul Mushlih is a genetics and molecular biology researcher who works at the Muhammadiyah University of Sidoarjo. Graduate from Gadjah Mada University. The research focuses on the optimization of molecular biology methods for the examination, detection of diseases and pathogens, and genetic markers of congenital disorders.

Fitri Kumala Sari, Department Medical Laboratory Technology, Universitas Muhammadiyah Sidoarjo, Sidoarjo, Jawa Timur, Indonesia

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Fitri Khumala Sari. She worked as a COVID-19 aid worker at the Surabaya Class 1 Port Health Office.

Hindah Sabrina Amin, Department Medical Laboratory Technology, Universitas Muhammadiyah Sidoarjo, Sidoarjo, Jawa Timur, Indonesia

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Hindah Sabrina Amin. She currently works at Asih Abyata Pasuruan's hospital.

Siti Asriani Iknan, Department Medical Laboratory Technology, Universitas Muhammadiyah Sidoarjo, Sidoarjo, Jawa Timur, Indonesia

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Siti Asriani Iknan. She working at the Bataraguru Public Health Center, Baubau City.

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