The effect of temperature and storage time of cuccal swabs on FGA and D13S317 loci with the STR PCR method

Main Article Content

Indah Nuraini Masjkur Evy Arfianti Ahmad Yudianto Abdul Hadi Furqoni Qurrota A’yun

Abstract

The samples used for forensic DNA analysis in living individuals are usually blood and buccal swabs, however, blood collection requires an invasive method that can cause discomfort, thus a buccal swab can be a good choice for individuals examined, especially children. This study aimed to determine the effect of temperature and storage time of buccal swabs on the quantity of DNA as material for DNA examination in the forensic field. This study was a laboratory experiment to determine the effect after treatment. Buccal swab samples were 48 and divided into 2 temperature groups, namely room temperature (RT) and 4℃. The division of the temperature groups was also observed with time differences, namely 1, 3, 5, 7 days. EDNA extraction used the DNAzol method and DNA quantification used a Spectrophotometer. The PCR process was carried out with STR primers FGA and D13S317 loci. The visualization stage used acrylamide gel and silver staining.

The results of this study prove that there is an effect of temperature and storage time of buccal swab samples. The longer the treatment time, the lower the DNA level. With statistical analysis, it is obtained p-value of <0.005, it can be concluded that there are significant differences in DNA levels at the temperature and storage time treatments of the buccal swab sample. The results of DNA visualization at the FGA and D13S317 loci using the STR PCR method in this study can still be detected and can be used as a reference for examination in forensic cases.

 

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How to Cite
Masjkur, I., Arfianti, E., Yudianto, A., Furqoni, A., & A’yun, Q. (2020). The effect of temperature and storage time of cuccal swabs on FGA and D13S317 loci with the STR PCR method. Jurnal Teknologi Laboratorium, 9(2), 192-198. https://doi.org/https://doi.org/10.29238/teknolabjournal.v9i2.243
Section
Clinical Chemistry
Author Biographies

Indah Nuraini Masjkur, Human Genetic Study Group Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia

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Indah Nuraini Masjur S.Si., M.Si was born in Surabaya by receiving forensic master tittle in 2011 from Airlangga University with a concentration in biological moleculer forensic. Now, she works as laboratory technician at Human Genetic Laboratory Institute of Tropical Disease Airlangga University

Evy Arfianti, Faculty of Public Health, Universitas Airlangga, Surabaya, Indonesia

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Evy Arfianti S.KM., M.Kes as a laboratory technician at Public Health Faculty Airlangga University

Ahmad Yudianto, Human Genetic Study Group Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia

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Dr. Ahmad Yudianto,dr,SpF,SH,M.Kes is Forensic Science and Medico-legal Specialist is a Lecturer and Head of the Forensic Science Master Program at the University of Airlangga since 2010/2011, and is a Specialist Doctor’s Education Program since 2015. Forensic, Medico-legal, and Expert Witness since 2007 and at Human Genetic Laboratory Institute of Tropical Disease Airlangga University.

Abdul Hadi Furqoni, Post Graduate Doctoral Program Degree Program, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

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Abdul Hadi Furqoni, MFS, BScN, PhD student in Medical Science at School of Medicine, and a Researcher at Human Genetic Laboratory Institute of Tropical Disease Airlangga University

Qurrota A’yun, Human Genetic Study Group Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia

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Qurrota A’yun S.KM as a laboratory technician at Human Genetic Laboratory Institute of Tropical Disease Airlangga University

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