1300
Abstract Views
735
PDF Download
Clinical Chemistry

Isolation and Identification of an Alkaloid Compound from Bebuas Leaves (Premna serratifolia) as an Anti-Inflammatory in White Rats (Rattus norvegicus)

Muhammad Irhash Shalihin Department of Mathematics and Natural Science, University of Jambi , Indonesia Correspondence m.irhash@unja.ac.id
,
Muhaimin Department of Pharmacy, University of Jambi, Indonesia Correspondence muhaimin.fkip@unja.ac.id
,
Madyawati Latief Department of Mathematics and Natural Science, University of Jambi, Indonesia Correspondence madyawatilatief@unja.ac.id
Pages 78-94
Browse this journal

Abstract

Bebuas (Premna serratifolia) traditionally is used by Malay people as a traditional medicine to restore health. This indicates it contains chemicals that potentially have an anti-inflammatory activity. The results of phytochemical screening showed that the ethanol extract contains phenolics, alkaloids, flavonoids, steroids and saponins. Meanwhile, hexane extract only contains steroids. Anti-inflammatory activity evaluation on crude extracts showed that the ethanol extract was more active than the hexane extract. Therefore, the isolation step was continued to ethanol extract with the targeted compound was an alkaloid. Extraction was conducted by using acid-base and partition techniques to give the alkaloids fraction (EaBW). EaBW is then further separated by liquid vacuum column technique using gradual polarity elution, with eluent of ethyl acetate (Ea) - methanol and obtained 5 fractions. The isolate was obtained from F.3. The isolate showed it had anti-inflammatory activity with ED50 = 5.45 mg/KgBW. The UV-Vis and FT-IR spectra patterns of the isolate have similarities with ciprofloxacin as well as its physical characteristics which are hygroscopic yellowish crystal, and fluorescence property. Therefore, the isolated compound is thought to have a similar structure to ciprofloxacin.

There is no Figure or data content available for this article

References

1. Vadivu R, Suresh A, Girinath K, Kannani P, Vimala R, Kumar N. Evaluation of Hepatoprotective and In-vitro Cytotoxic Activity of Leaves of Premna serratifolia Linn. J Sci Res. 2009;1:145-152. doi:10.3329/jsr.v2i3.4899
2. Saim. Pendayangunaan Sumber Daya Hutan Bagi Suku Talang Mamak di Daerah Seberrida Riau. Pros Semin Nas dan Lokarya Etnobotani 1 Cisar Bogor. 1992:381-389.
3. Padua LS de, Bunyapraphatsara N, Lemmens RHMJ. Plant Resources of South-East Asia 12 (3): Medicinal and Poisonous Plants 3. Vol 87. Bogor: Backhuys Publishers; 1999. doi:10.1016/s0378-8741(03)00056-4
4. Parawansah, Nuralifah, Akib N, Antrie G. Uji Toksisitas Akut Ekstrak Etanol Daun Buas-Buas (Premna serratifolia Linn.) Terhadap Larva Udang (Artemia salina Leach) dengan Metode Brine Shrimplethality Test (BLST). 2017;(April):171-177.
5. Katzung BG, Masters SB, Trevor AJ. Basic and Clinical Pharmacology. 12th ed. New York: Mc Graw Hill; 2012.
6. Adyttia A, Untari EK, Wahdaningsih S. Efek Ekstrak Etanol Daun Premna cordifolia terhadap Malondialdehida Tikus yang Dipapar Asap Rokok. Pharm Sci Res. 2017;1(2):104-115. doi:10.7454/psr.v1i2.3302
7. Setyaningrum PA. Isolasi dan Identifikasi Senyawa Fenolik dari Fraksi Etil Asetat Kulit Batang Tumbuhan Turi (Sesbania grandiflora) serta Uji Bioaktivitas Antibakteri. Skripsi Univ Lampung. 2016.
8. Ikawati Z. Farmakoterapi Penyakit Sistem Saraf Pusat. Yogyakarta: Bursa Ilmu; 2011.
9. Black PH, Garbutt LD. Stress, inflammation and cardiovascular disease. J Psychosom Res. 2002;52(1):1-23. doi:10.1016/S0022-3999(01)00302-6
10. World Health Expectancy. Indonesia: Life Expectancy. World Health Expectancy. https://www.worldlifeexpectancy.com/indonesia-life-expectancy. Published 2018. Accessed December 10, 2019.
11. Tjay TH, Rahardja K. Obat-Obat Penting Khasiat, Penggunaan Dan Efek-Efek Sampingnya. 6th ed. Jakarta: PT. Elex Media Komputindo; 2007.
12. Harbone JB. Metode Fitokimia : Penuntun Cara Modern Menganalisis Tumbuhan. 2nd ed. (Padmawinata K, Soediro I, eds.). Bandung: ITB Press; 1987.
13. Titis M, Fachriyah E, Kusrini D. Isolasi, Identifikasi dan Uji Aktifitas Senyawa Alkaloid Daun Binahong (Anredera cordifolia (Tenore) Steenis). Chem Info J. 2013;1(1):196-201.
14. Emrizal, Fernando A, Suryani F, Ahmad F, Sirat HM, Dayar Arbain D. Isolasi senyawa dan uji aktivitas anti-inflammasi Isolasi Senyawa dan Uji Aktivitas Anti-inflammasi Ekstrak Metanol Daun Puwar Kincung (Nicolaia speciosa Horan). J Penelit Farm Indones. 2012;1(1):1-5.
15. Hasanah F, Hidayah N. Uji Aktivitas Antiinflamasi Ekstrak Air Daun Salam (Sygyzium polyanthum Wight.) Terhadap Tikus Wistar Jantan yang Diinduksi dengan Karagenan 1%. J Pharm Sci. 2018;1(1):16-22.
16. Apridamayanti P, Sanera F, Robiyanto R. Aktivitas Antiinflamasi Ekstrak Etanol Daun Karas ( Aquilaria malaccensis Lamk .) Antiinflammatory Activity of Ethanolic Extract from Karas Leaves ( Aquilaria malaccensis Lamk .). 2018;5(3):152-158.
17. Luliana, S., Susanti, R. dan Agustina E. Uji Aktivitas Antiinflamasi Ekstrak Air Herba Ciplukan (Physalis angulata L.) terhadap Tikus Putih (Rattus norvegicus L.) Jantan Galur Wistar yang diinduksi Karagenan. Maj Obat Tradis. 2017;22(3):199. doi:10.22146/mot.31556
18. Ross GJS, Finney DJ. Probit Analysis. Stat. 1972;21(3):222. doi:10.2307/2986688
19. Aslamiah S, Haryadi. Identifikasi Kandungan Kimia Golongan Senyawa daun pohon kapuk. Anterior J. 2014;53(9):1689-1699. doi:10.1017/CBO9781107415324.004
20. Oktaviani E, Wibowo MA, Idiawati N. Penapisan Fraksi Antioksidan Daun Buas-buas ( Premna serratifolia Linn ). J Kim Khatulistiwa. 2015;4(3):40-47.
21. Dianita R, Jantan I. Ethnomedicinal uses, phytochemistry and pharmacological aspects of the genus Premna: A review. Pharm Biol. 2017;55(1):1715-1739. doi:10.1080/13880209.2017.1323225
22. Wibowo S, Gofir A. Farmakoterapi Nyeri Inflamasi: Farmakoterapi Dalam Neurologi. 1st ed. Jakarta: Salemba Medika; 2001.
23. Vandamme TF. Use of Rodents as Models of Human Diseases. 2014;6(1):2-9. doi:10.4103/0975-7406.124301
24. Dachriyanus. Analisis Struktur Senyawa Organik Secara Spektroskopi. Padang: Lembaga Pengembangan Teknologi Informasi dan Komunikasi (LPTIK) Universitas Andalas; 2004.
25. Abdou MM. Chemistry of 4-Hydroxy-2 ( 1 H ) -quinolone . Part 1 : Synthesis and reactions. Arab J Chem. 2018;10(December):S3324-S3337. doi:10.1016/j.arabjc.2014.01.012
26. Khan ZH, Aziz A, Hasan R, Al-mamun R. The role of drug as corrosion inhibitor for mild steel surface characterization by SEM , AFM , and FTIR. 2016;(May 2017). doi:10.1108/ACMM-11-2015-1597
27. Sunitha P, Amareshwar P, Kumar MS, Chakravarti P. Study on Effect of Solvents & Nonsolvents on Microspheres of Ciprofloxacin: Coacervation Phase Separation. 2015;(January 2010).
28. Pagare S, Bhatia M, Tripathi N, Pagare S, Bansal YK. Secondary metabolites of plants and their role: Overview. Curr Trends Biotechnol Pharm. 2015;9(3):293-304.
29. Zuyun H, Houping H, Ruxiu C, Yun Z. Study on the Fluorescence Properties of Some Fluoroquinolones in Various Media. 1997;2(3):2-7.
30. Malacara D. Color Vision and Colorimetry: Theory and Applications. New York: SPIE Press; 2002.
There is no Supplemental content for this article.

How to Cite This

Shalihin, M. I., Muhaimin, & Latief, M. (2022). Isolation and Identification of an Alkaloid Compound from Bebuas Leaves (Premna serratifolia) as an Anti-Inflammatory in White Rats (Rattus norvegicus). Jurnal Teknologi Laboratorium, 11(2), 78–94. https://doi.org/10.29238/teknolabjournal.v11i2.275

Article Metrics

Download Statistics

Downloads

Download data is not yet available.

Other Statistics

Verify authenticity via CrossMark

Copyright and Permissions

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Publishing your paper with Jurnal Teknologi Laboratorium (JTL) means that the author or authors retain the copyright in the paper. JTL granted an exclusive reuse license by the author(s), but the author(s) are able to put the paper onto a website, distribute it to colleagues, give it to students, use it in your thesis etc, even commercially. The author(s) can reuse the figures and tables and other information contained in their paper published by JTL in future papers or work without having to ask anyone for permission, provided that the figures, tables or other information that is included in the new paper or work properly references the published paper as the source of the figures, tables or other information, and the new paper or work is not direct at private monetary gain or commercial advantage.

JTL journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. This journal is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License. This license lets others remix, transform, and build upon the material for any purpose, even commercially.

JTL journal Open Access articles are distributed under this Creative Commons Attribution-ShareAlike 4.0 International License (CC BY-SA). Articles can be read and shared for All purposes under the following conditions:

  • BY: You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
  • SA:  If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.

Data Availability

All data generated during this study are included in this published article [and its supplementary information files].