Abstract

Tuberculosis (TB) is one of the infectious diseases that have become a major problem in Indonesia. This disease is caused by the Mycobacterium tuberculosis bacteria. The bacteria are commonly treated with antibiotics. However, the use of irrelevant antibiotics is the most influential factor of antibiotic resistance. Therefore, natural ingredients are required as novel antibiotic agents, some of which are sourced from lactic acid bacteria. This study aims at investigating the antituberculosis activities of Pediococcus acidilactici and Lactobacillus plantarum isolated from breast milk against M. tuberculosis. Pediococcus acidilactici and Lactobacillus plantarum breast milk isolates were rejuvenated with MRS (De Man, Rogosa and Sharpe) broth media, and the supernatant was then neutralized to pH 7.0 using a pH meter by adding 1N NaOH solution. Antituberculosis activity test was performed with Lowensten Jensen media to investigate the growth of M. tuberculosis. The results of this study showed that Pediococcus acidilactici had antituberculosis activity at 48 hours at concentrations of 80% and 90%, while the secondary metabolites of L. plantarum had antituberculosis activity at 24 hours and 48 hours at concentrations of 60%, 70%, 80%, 90%. Therefore, this study concludes that P. acidilactici and L. plantarum bacteria have the potential to be developed as antituberculosis agents.

There is no Figure or data content available for this article

References

• Worldometer. TB Update. Published online 2021.
• Erah P, Ojieabu W. Success of the control of tuberculosis in Nigeria: a review. Int J Heal Res. 2010;2(1):3-14. doi:10.4314/ijhr.v2i1.55382 (http://www.ajol.info/index.php/ijhr/article/view/55382)
• Fogel N. Tuberculosis: A disease without boundaries. Tuberculosis. 2015;95(5):527-531. doi:10.1016/j.tube.2015.05.017 (https://linkinghub.elsevier.com/retrieve/pii/S1472979214206950)
• Meena. R and LS. Unique Characteristic Features of Mycobacterium Tuberculosis in Relation to Immune System. Am J Immunol. 2011;7(1):1-8.
• Palomino J, Martin A. Drug Resistance Mechanisms in Mycobacterium tuberculosis. Antibiotics. 2014;3(3):317-340. doi:10.3390/antibiotics3030317(http://www.mdpi.com/2079-6382/3/3/317)
• Igarashi M. Development of new antituberculosis drugs from natural products†. Biosci Biotechnol Biochem. 2017;81(1):32-37. doi:10.1080/09168451.2016.1248369 (https://academic.oup.com/bbb/article/81/1/32/5938808)
• Zacharof MP, Lovitt RW. Bacteriocins Produced by Lactic Acid Bacteria a Review Article. APCBEE Procedia. 2012;2:50-56. doi:10.1016/j.apcbee.2012.06.010 (https://linkinghub.elsevier.com/retrieve/pii/S2212670812000814)
• Sharma G, Dang S, Gupta S, Gabrani R. Antibacterial Activity, Cytotoxicity, and the Mechanism of Action of Bacteriocin from Bacillus subtilis GAS101. Med Princ Pract. 2018;27(2):186-192. doi:10.1159/000487306 (https://www.karger.com/Article/FullText/487306)
• Aguilar-Pérez C, Gracia B, Rodrigues L, et al. Synergy between Circular Bacteriocin AS-48 and Ethambutol against Mycobacterium tuberculosis. Antimicrob Agents Chemother. 2018;62(9):1-13. doi:10.1128/AAC.00359-18 (https://journals.asm.org/doi/10.1128/AAC.00359-18)
• Chen MM, Liu QH, Xin GR, Zhang JG. Characteristics of lactic acid bacteria isolates and their inoculating effects on the silage fermentation at high temperature. Lett Appl Microbiol. 2013;56(1):71-78. doi:10.1111/lam.12018 (https://onlinelibrary.wiley.com/doi/10.1111/lam.12018)
• Kim SY, Yi DY. Analysis of the human breast milk microbiome and bacterial extracellular vesicles in healthy mothers. Exp Mol Med. 2020;52(8):1288-1297. doi:10.1038/s12276-020-0470-5 (https://www.nature.com/articles/s12276-020-0470-5)
• Rodríguez JM. The Origin of Human Milk Bacteria: Is There a Bacterial Entero-Mammary Pathway during Late Pregnancy and Lactation? Adv Nutr. 2014;5(6):779-784. doi:10.3945/an.114.007229 (https://academic.oup.com/advances/article/5/6/779/4558090)
• Osmanagaoglu, Ozlem., Ingolf Nes., and Fadime K. A probiotic bacterium Pediococcus pentosaceus OZF, Isolated from Human Breast Milk Produces Pediocin AcH/PA-1. African J Biotechnol. 2011;10(11):2070-2079. https://www.ajol.info/index.php/ajb/article/view/93115
• Lestari SD, Sadiq ALO, Safitri WA, Dewi SS, Prastiyanto ME. The antibacterial activities of bacteriocin Pediococcus acidilactici of breast milk isolate to against methicillin-resistant Staphylococcus aureus. J Phys Conf Ser. 2019;1374(1):012021. doi:10.1088/1742-6596/1374/1/012021 (https://iopscience.iop.org/article/10.1088/1742-6596/1374/1/012021)
• Lin T-H, Pan T-M. Characterization of an antimicrobial substance produced by Lactobacillus plantarum NTU 102. J Microbiol Immunol Infect. 2019;52(3):409-417. doi:10.1016/j.jmii.2017.08.003 (https://linkinghub.elsevier.com/retrieve/pii/S1684118217301846)
• Hassan MU, Nayab H, Rehman TU, et al. Characterisation of Bacteriocins Produced by Lactobacillus spp. Isolated from the Traditional Pakistani Yoghurt and Their Antimicrobial Activity against Common Foodborne Pathogens. Biomed Res Int. 2020;2020:1-10. doi:10.1155/2020/8281623 (https://www.hindawi.com/journals/bmri/2020/8281623/)

How to Cite This

Article Metrics

Download Statistics

Downloads

Download data is not yet available.

Other Statistics

Copyright and Permissions

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Publishing your paper with Jurnal Teknologi Laboratorium (JTL) means that the author or authors retain the copyright in the paper. JTL granted an exclusive reuse license by the author(s), but the author(s) are able to put the paper onto a website, distribute it to colleagues, give it to students, use it in your thesis etc, even commercially. The author(s) can reuse the figures and tables and other information contained in their paper published by JTL in future papers or work without having to ask anyone for permission, provided that the figures, tables or other information that is included in the new paper or work properly references the published paper as the source of the figures, tables or other information, and the new paper or work is not direct at private monetary gain or commercial advantage.

JTL journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. This journal is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License. This license lets others remix, transform, and build upon the material for any purpose, even commercially.

JTL journal Open Access articles are distributed under this Creative Commons Attribution-ShareAlike 4.0 International License (CC BY-SA). Articles can be read and shared for All purposes under the following conditions:

• BY: You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
• SA:  If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.

Data Availability