In this publication, we have developed a simple and quick isolation method of stem cells from the umbilical cord. Usually, the umbilical cord is a waste product during the child delivery procedure, but the umbilical cords contain valuable stem cells called mesenchymal stroma stem cells (also called MSCs), which can be the basis for different applications e.g., testing the effect of therapeutic compounds as well as the development of therapeutic applications for the cure of degenerative diseases like bone and cartilage related. These stem cells can be used to create new artificial organs too. The umbilical cord was cut with sterile scissors into small pieces (1-2 mm size) and these pieces were placed on 6 well culture plates. The cell culture media was added to these plates after 5-10 minutes placing these plates in a CO2 incubator. The successful growth of cells was observed after 7 days in wells, where pieces of umbilical cord were implanted. Microscopic observations show how the cells are oozing out from these pieces. These cells were isolated and passaged further. These adhere to the plastic surface, were fibroblast shaped, and were isolated successfully in different experiments.
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- Atala A., Lanza R., Thomson J.A. and Nerem R. (2011) Principles of Regenerative Medicine, Second Edition.. Elsevier; USA. DOI:10.1016/C2009-0-61040-0
- Bari D.C., Dell´Accio F., Tylzanowski P. and Luyten P. F. (2001) Multipotent Mesenchymal Stem Cells From Adult Synovial Membrane. Arthritis & Rheumatism. 44(8). 1928-1942. https://doi.org/10.1002/1529-0131(200108)44:8<1928::AID-ART331>3.0.CO;2-P
- Bartolo D. L. and Bader A. (2013) BIOMATERIAL AND STEM CELL THERAPY State of Art and Vision for the Future. CRC Press. USA.
- Beeravolu N., McKee C., Alamari A., Mikhael S., Brown C., Perez-Cruet M. and Chaudhry G.R. (2017) Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta. JoVe Journal. DOI: 10.3791/55224
- Bhatia, S. (2021). A simple method for isolation of rest of trypsinized stem cells with magnetic beads. Jurnal Teknologi Laboratorium, 10(1), 01-02. DOI:10.29238/teknolabjournal.v10i1.268
- Carter K, Lee HJ, Na KS, Fernandes-Cunha GM, Blanco IJ, Djalilian A, Myung D. Characterizing the impact of 2D and 3D culture conditions on the therapeutic effects of human mesenchymal stem cell secretome on corneal wound healing in vitro and ex vivo.
- Acta Biomater. 2019 Nov;99:247-257. doi: 10.1016/j.actbio.2019.09.022. Epub 2019 Sep 17. PMID: 31539656; PMCID: PMC7101245.
- Friedenstein A.J., Chailakhyan R.K. and Gerasimov U.V. (1987) Bone marrow osterogenic stem cells in vitro cultivation and transplantation in different chambers. Cell Tissue Kinet. 20(3) 263-272.
- Friedenstein A.J., Latrinik N.W., Grosbeva A.C. and Gonkaya U.B. (1982) Marrow microevironment transfer by heterotopic transplantation of freshly isolated and cultured cells in porous sponges. Exp. Hematog. 10(2). 217-227.
- Gazit Z., Pelled G., Sheyn D., Kimelman N. and Gazit D. (2011). Mesenchymal Stem Cells. Principles of Regenerative Medicine, Second Edition. Elsevier. USA. 17, 285-304.
- Kay, A.G., Long, G., Tyler, G. et al. Mesenchymal Stem Cell-Conditioned Medium Reduces Disease Severity and Immune Responses in Inflammatory Arthritis. Sci Rep 7, 18019 (2017). https://doi.org/10.1038/s41598-017-18144-w
- Mishra, S., Sevak, J.K., Das, A. et al. Umbilical cord tissue is a robust source for mesenchymal stem cells with enhanced myogenic differentiation potential compared to cord blood. Sci Rep 10, 18978 (2020). https://doi.org/10.1038/s41598-020-75102-9
- Stefa?ska, Katarzyna, Sibiak, Rafa?, Dompe, Claudia, Moncrieff, Lisa, Hutchings, Greg, Janowicz, Krzysztof and Kempisty, Bartosz. "Overview of methods of isolation, cultivation and genetic profiling on human umbilical cord stem cells" Medical Journal of Cell Biology, vol.7, no.4, 2019, pp.170-174. https://doi.org/10.2478/acb-2019-0023
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