A simple and quick isolation method to obtain a huge number of fibroblasts like mesenchymal stem cells from human umbilical cord
In this publication, we have developed a simple and quick isolation method of stem cells from the umbilical cord. Usually, the umbilical cord is a waste product during the child delivery procedure, but the umbilical cords contain valuable stem cells called mesenchymal stroma stem cells (also called MSCs), which can be the basis for different applications e.g., testing the effect of therapeutic compounds as well as the development of therapeutic applications for the cure of degenerative diseases like bone and cartilage related. These stem cells can be used to create new artificial organs too. The umbilical cord was cut with sterile scissors into small pieces (1-2 mm size) and these pieces were placed on 6 well culture plates. The cell culture media was added to these plates after 5-10 minutes placing these plates in a CO2 incubator. The successful growth of cells was observed after 7 days in wells, where pieces of umbilical cord were implanted. Microscopic observations show how the cells are oozing out from these pieces. These cells were isolated and passaged further. These adhere to the plastic surface, were fibroblast shaped, and were isolated successfully in different experiments.
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- Friedenstein AJ, Chailakhyan RK, Gerasimov UV. Bone marrow osteogenic stem cells: in vitro cultivation and transplantation in diffusion chambers. Cell Prolif. 1987;20(3):263-272. doi:10.1111/j.1365-2184.1987.tb01309.x
- Friedenstein AJ, Latzinik NW, Grosheva AG, Gorskaya UF. Marrow microenvironment transfer by heterotopic transplantation of freshly isolated and cultured cells in porous sponges. Exp Hematol. 1982;10(2):217-227.
- Mishra S, Sevak JK, Das A. Umbilical cord tissue is a robust source for mesenchymal stem cells with enhanced myogenic differentiation potential compared to cord blood. Sci Rep. 2020;10:18978. doi:10.1038/s41598-020-75102-9
- Bari D.C. TP Dell ?Accio F, F. LP. Multipotent Mesenchymal Stem Cells From Adult Synovial Membrane. Arthritis Rheum. 2001;44(8). doi:10.1002/1529-0131(200108)44:8
- Atala A, Lanza R, Thomson JA, Nerem R. Principles of Regenerative Medicine. Elsevier; 2011. doi:10.1016/C2009-0-61040-0
- Gazit Z, Pelled G, Sheyn D, Yakubovich DC, Gazit D. Principles of regenerative medicine. Published online 2019.
- De Bartolo L, Bader A, eds. Biomaterials for Stem Cell Therapy: State of Art and Vision for the Future. CRC Press; 2013.
- Bhatia S. A simple method for isolation of rest of trypsinized stem cells with magnetic beads. J Teknol Lab. 2021;10(1):01-02. doi:10.29238/teknolabjournal.v10i1.268
- Kay AG, Long G, Tyler G. Mesenchymal Stem Cell-Conditioned Medium Reduces Disease Severity and Immune Responses in Inflammatory Arthritis. Sci Rep. 2017;7:18019. doi:10.1038/s41598-017-18144-w
- Carter K, Lee HJ, Na KS, et al. Characterizing the impact of 2D and 3D.
- N. B, C. M, A. A, et al. Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta. JoVe J. Published online 2017. doi:10.3791/55224
- Stefa?ska K, Sibiak R, Dompe C, et al. Overview of methods of isolation, cultivation and genetic profiling on human umbilical cord stem cells. Med J Cell Biol. 2019;7(4):170-174. doi:10.2478/acb-2019-0023
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