779
Abstract Views
PDF Download
Hematology

The potential of Aloe vera gel as an alternative inductor in platelet aggregation test: .

,

Abstract

Platelet aggregation test is important in evaluation platelet function. Inductors are substances that stimulate the aggregation process. The use of ADP (adenosine diphosphate as an inductor has the disadvantage of being costly and difficult to obtain. Aloe vera is a plant from Africa which has been widely used for wound healing, therefore has the potential as an alternative inductor agent. This study aims to elucidate the effectiveness of Aloe vera gel as a platelet inductor compared to ADP reagent with the Velaskar method. This research started with the preparation of fresh Aloe vera to obtain Aloe vera gel. Then, venous blood was taken from 16 participants for platelet aggregation test. Platelet aggregation test was divided into two groups, namely ADP group (addition of ADP) and Aloe vera group (addition of aloe vera gel). The last step was analyze the results of platelet aggregation and statistically using the Independent Sample T-test. The average of percentage platelet aggregation with ADP and Aloe vera gel was 88.2 ± 5.6% and 85.7 ± 5.4%, respectively. Independent sample T-test analysis showed no significant difference between the percentage of platelet aggregation with ADP and Aloe vera gel. In conclusion, Aloe vera gel has the potential as an alternate platelet inductor. Some of the benefits of aloe vera gel are the ability to induce platelets as well as ADP, the application does not affect the morphology of erythrocytes, economical and practical (does not require elaborate preparation). Although the disadvantage is pure Aloe vera gel includes several components that able affect the performance of Aloe vera gel as an inductor.

There is no Figure or data content available for this article

References

  • Sierra C, Moreno M, García-Ruiz JC. 2022. The physiology of hemostasis. Blood Coagul Fibrinolysis. 1(33) (Suppl 1):S1-S2. doi: 10.1097/MBC.0000000000001099
  • Pagana, K. D., Pagana, T. J. 2014. Mosby's Manual of Diagnostic and Laboratory Tests Fifth Edition. Elsevier.
  • Black, J., Hawks, J. 2014. Keperawatan Medikal Bedah: Managemen Klinis untuk Hasil yang Diharapkan. Edisi 8. Singapore: Elsevier.
  • Riskesdas (Riset Kesehatan Dasar). 2018. Badan Penelitian Dan Pengembangan Kesehatan Kementerian Kesehatan RI. diakses pada tanggal 10 Juni 2022
  • Benjamin EJ., Blaha MJ., Chiuve SE., Cushman M., Das SR., Deo R., de Ferranti SD., Floyd J., Fornage M., Gillespie C., Isasi CR., Jimenez MC., Jordan LC., Judd SE., Lackland D., Lichtman JH., Lisabeth L., Liu S,. Longenecker CT., Mackey RH., Matsushita K., Mozaffarian D., Mussolino ME., Nasir K., Neumar RW., Palaniappan L., Pandey DK., Thiagarajan RR, Reeves MJ., Ritchey M., Rodriguez CJ., Roth GA., Rosamond WD., Sasson C., Towfighi A., Tsao CW., Turner MB., Virani SS., Voeks JH., Willey JZ., Wilkins JT., Wu JHY., Alger HM., Wong SS., Munthner P. 2017. Heart Disease and Stroke Statistics—2017 Update: A Report From the American Heart Association. Circulation. 135(10):e146–e603. https://doi.org/10.1161/CIR.0000000000000485
  • Velaskar, DS., Chitre, AP. 1982. A New Aspect of Platelet Aggregation and a Test to Measure It. American Journal of Clinical Pathology. 77(3):267–274. doi:10.1093/ajcp/77.3.267
  • Kehrel BE, Brodde MF. 2013. State of the art in platelet function testing. Transfus Med Hemother.40:73–86.
  • Koltai, K., Kesmarky G., Feher G., Tibold A., Toth K. 2017. Platelet Aggregometry Testing: Molecular Mechanisms, Techniques and Clinical Implications. International Journal of Molecular Sciences. 18(8):1803 doi:10.3390/ijms18081803
  • Harumi A, Ade Z. 2016. Review: Aktivitas Tanaman Lidah Buaya (Aloe vera Linn) sebagai Penyembuh Luka. Farmaka: peer-reviewed. 15(2): 82-89
  • Marzanna H, Krzysztof D, Danuta G, Anna J, Elzbieta G. 2019. Aloe vera (L.) Webb: Natural Sources of Antioxidants – A Review. Springer: Plant Foods Hum Nutr. 19(6):1-11. doi: 10.1007/s11130-019-00747-5
  • Sukeksi, A., Rizqi YR. 2021. Potensi Ekstrak Etanol Daun Sirih (Piper Betle L.) Sebagai Pengganti ADP (Adenosine Diphosphate) Pada Pemeriksaan Agregasi Trombosit. Lontara: Journal of Health Science & Technology. 2(1):11-17
  • Septiani, Siti FM., Gemala A. 2020. Aktivitas Antioksidan Dan Kadar Aloin Pada Lidah Buaya (Aloe Vera Chinensis). Jurnal Medika Indonesia. 1 (2): 17-23.
  • Heng, HC., Mohd HZ., Pei YN. (2018). Pharmaceutical Applications of Aloe vera. Indonesian J. Pharm. 29(3): 101-116 DOI: 10.14499/indonesianjpharm29iss3pp101
  • Sianturi, CY. 2019. Manfaat Lidah Buaya Sebagai Anti Penuaan Melalui Aktivitas Antioksidan. ESSENTIAL:Essence of Scientific Medical Journal. 17(1):34-38
  • Zagórska-Dziok, M., Furman-Toczek D., Dudra-Jastrz?bska M., Zygo K., Stanis?awek A., Kapka-Skrzypczak L. 2017. Evaluation of clinical effectiveness of Aloe vera – a review. J Pre-Clin Clin Res. 11(1): 86–93. doi: 10.26444/jpccr/74577
  • Koupenova, M., Clancy L., Corkrey HA., Freedman JE. 2018. Circulating Platelets as Mediators of Immunity, Inflammation, and Thrombosis. Circulation Research. 122(2): 337–351. doi:10.1161/CIRCRESAHA.117.310795
  • Shakib, Z., Shahraki NR., Bibi M., Hosseinzadeh H. 2019. Aloe vera as an herbal medicine in the treatment of metabolic syndrome: A review. Phytotherapy Research. 33(10). 2649–2660. doi:10.1002/ptr.6465
  • Koltai, K., Kesmarky G., Feher G., Tibold A., Toth K. (2017). Platelet Aggregometry Testing: Molecular Mechanisms, Techniques and Clinical Implications. International Journal of Molecular Sciences. 18(8):1803 doi:10.3390/ijms18081803
  • Fontana, P., Zufferey A., Daali Y., Reny J.L. (2014). Antiplatelet therapy: Targeting the TxA2 pathway. J. Cardiovasc. Transl. Res. 7(2014):29–38 DOI:https://doi.org/10.1007/s12265-013-9529-1
  • Paniccia, R., Priora R., Alessandrello LG., Abbate R. 2015. Platelet function tests: a comparative review. Vascular Health and Risk Management. 2015(11):133-148. doi:10.2147/VHRM.S44469
  • Harrison, P., Ian M., Andrew M., Carol B., Ri L., Mark Winter; Sam Machin. 2011. Guidelines for the laboratory investigation of heritable disorders of platelet function. British Committee for Standards in Haematology. 155(1):30–44. doi:10.1111/j.1365-2141.2011.08793.x
  • Maleki, SJ., Crespo JF., Cabanillas B. (2019). Anti-inflammatory effects of flavonoids. Food Chemistry. 299(2019):125124. doi:10.1016/j.foodchem.2019.125124
There is no Supplemental content for this article.

How to Cite This

Putri, G. S. A., & Sukeksi, A. (2023). The potential of Aloe vera gel as an alternative inductor in platelet aggregation test: . Jurnal Teknologi Laboratorium, 12(1). https://doi.org/10.29238/teknolabjournal.v12i1.392

Article Metrics

Download Statistics

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Other Statistics

Verify authenticity via CrossMark

Copyright and Permissions

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Publishing your paper with Jurnal Teknologi Laboratorium (JTL) means that the author or authors retain the copyright in the paper. JTL granted an exclusive reuse license by the author(s), but the author(s) are able to put the paper onto a website, distribute it to colleagues, give it to students, use it in your thesis etc, even commercially. The author(s) can reuse the figures and tables and other information contained in their paper published by JTL in future papers or work without having to ask anyone for permission, provided that the figures, tables or other information that is included in the new paper or work properly references the published paper as the source of the figures, tables or other information, and the new paper or work is not direct at private monetary gain or commercial advantage.

JTL journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. This journal is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License. This license lets others remix, transform, and build upon the material for any purpose, even commercially.

JTL journal Open Access articles are distributed under this Creative Commons Attribution-ShareAlike 4.0 International License (CC BY-SA). Articles can be read and shared for All purposes under the following conditions:

  • BY: You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
  • SA:  If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.

Data Availability

 
Keywords