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Histology

Decreasing alpha-synuclein aggregation on rotenone-induced adult Zebrafish as Parkinson’s Diseases model by ethanol extraction of Keluwih (Artocarpus camansi) leaves

Marisca Evalina Gondokesumo Universitas Surabaya, Indonesia Correspondence marisca@staff.ubaya.ac.id
,
Faisal Akhmal Muslikh Universitas Airlangga, Indonesia Correspondence marisca@staff.ubaya.ac.id
,
Ni Putu Diah Nopitasari Univeersitas Surabaya, Indonesia Correspondence marisca@staff.ubaya.ac.id
,
Putu Dea Angelita Putri Universitas Airlangga, Indonesia Correspondence marisca@staff.ubaya.ac.id
Pages 53-60
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Abstract

The prevalence of Parkinson's disease is increasing every year. This progressive disease is characterized by the loss of neurons in the substantia nigra due to the presence of alpha-synuclein aggregates. Keluwih leaves (Artocarpus camansi) are known to have activity in inhibiting acetylcholinesterase, as well as being an antioxidant and anti-inflammatory. The aim of this study was to evaluate the effect of ethanol extract of A. camansi leaves on the levels of alpha-synuclein in male and female adult zebrafish induced with rotenone. The zebrafish were induced with rotenone at a concentration of 5 µg/L for 28 days, along with the administration of 96% ethanol extract of A. camansi leaves at doses of 2.5, 5, 7.5, or 10 mg/L. The media was changed every 48 hours to maintain the concentration of rotenone and extract. After 28 days, alpha-synuclein levels were examined using immunohistochemistry. The administration of ethanol extract of A. camansi leaves can reduce the average levels of alpha-synuclein in male and female adult zebrafish, with the optimum dose being 2.5 mg/L. Therefore, it can be concluded that the administration of ethanol extract of A. camansi leaves can be used as an alternative treatment for Parkinson's disease.

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References

  • World Health Organization. Parkinson Disease: A public health approach. World Health Organization. 2022;291(3):390.
  • Donadio V, Incensi A, Rizzo G, et al. The Effect of Curcumin on Idiopathic Parkinson Disease: A Clinical and Skin Biopsy Study. J Neuropathol Exp Neurol. 2022;81(7):545-552. doi:10.1093/jnen/nlac034
  • Robea MA, Balmus IM, Ciobica A, et al. Parkinson’s disease-induced Zebrafish models: Focussing on oxidative stress implications and sleep processes. Oxid Med Cell Longev. 2020;2020:1-15. doi:10.1155/2020/1370837
  • Razali K, Othman N, Mohd Nasir MH, et al. The promise of the zebrafish model for Parkinson’s disease: Today’s science and tomorrow’s treatment. Front Genet. 2021;12. doi:10.3389/fgene.2021.655550
  • Binienda ZK, Sarkar S, Mohammed-Saeed L, et al. Chronic exposure to rotenone, a dopaminergic toxin, results in peripheral neuropathy associated with dopaminergic damage. Neurosci Lett. 2013;541:233-237. doi:10.1016/J.NEULET.2013.02.047
  • Ma’arif B, Maimunah S, Muslikh FA, et al. Efek ekstrak daun Marsilea crenata Presl. pada aktivitas lokomotor ikan zebra. FARMASIS: Jurnal Sains Farmasi. 2022;3(1):18-24. doi:10.36456/FARMASIS.V3I1.5389
  • Oliveira RF. Mind the fish: zebrafish as a model in cognitive social neuroscience. Front Neural Circuits. 2013;7:1-15. doi:10.3389/fncir.2013.00131
  • Martinez TN, Greenamyre JT. Toxin models of mitochondrial dysfunction in Parkinson’s disease. Antioxid Redox Signal. 2012;16(9):920-934. doi:10.1089/ars.2011.4033
  • Prakash O, Kumar A, Gupta R. Evaluation of anticonvulsant activity of Artocarpus heterophyllus Lam. leaves (Jackfruit) in mice. Scholars Research Library. 2013;5(1):217-220. Accessed August 26, 2023. www.scholarsresearchlibrary.com
  • Jagtap UB, Bapat VA. Artocarpus: A review of its traditional uses, phytochemistry and pharmacology. J Ethnopharmacol. 2010;129(2):142-166. doi:10.1016/j.jep.2010.03.031
  • Solichah AI, Anwar K, Rohman A, Fakhrudin N. Profil fitokimia dan aktivitas antioksidan beberapa tumbuhan genus Artocarpus di Indonesia. JFood PharmSci. 2021;9(2):443-460. www.journal.ugm.ac.id/v3/JFPA
  • Das S, Laskar MA, Sarker SD, et al. Prediction of anti-Alzheimer’s activity of flavonoids targeting acetylcholinesterase in silico. Phytochemical Analysis. 2017;28(4):324-331. doi:10.1002/pca.2679
  • Outeiro TF, Ferreira JJ. Zebrafish as an Animal Model for Drug Discovery in Parkinson ’ s Disease and Other Movement Disorders?: A Systematic Review. 2018;9(June). doi:10.3389/fneur.2018.00347
  • Khotimah H, Ali M, Sumitro SB, Widodo MA. Decreasing ?-synuclein aggregation by methanolic extract of Centella asiatica in zebrafish Parkinson’s model. Asian Pac J Trop Biomed. 2015;5(11):948-954. doi:10.1016/j.apjtb.2015.07.024
  • Irwin MH, Parameshwaran K, Pinkert CA. Mouse models of mitochondrial complex I dysfunction. Int J Biochem Cell Biol. 2013;45(1):34-40. doi:10.1016/j.biocel.2012.08.009
  • Ott K. Rotenone. A brief review of its chemistry, environmental fate, and the toxicity of rotenone formulations. Environmental Science. Published online 2006.
  • Fleisch VC, Fraser B, Allison WT. Investigating regeneration and functional integration of CNS neurons: Lessons from zebrafish genetics and other fish species. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 2011;1812(3):364-380. doi:10.1016/j.bbadis.2010.10.012
  • Khotimah H, Sumitro SB, Aris Widodo M. Zebrafish Parkinson’s Model: Rotenone Decrease Motility, Dopamine, and Increase ?-Synuclein Aggregation and Apoptosis of Zebrafish Brain. Vol 8.; 2015.
  • Sherer TB, Betarbet R, Testa CM, et al. Mechanism of toxicity in rotenone models of Parkinson’s disease. The Journal of Neuroscience. 2003;23(34):10756-10764. doi:10.1523/JNEUROSCI.23-34-10756.2003
  • Uversky VN, Eliezer D. Biophysics of Parkinson’s Disease: Structure and Aggregation of ?-Synuclein. Vol 10.; 2009.
  • He Q, Song N, Xu H, Wang R, Xie J, Jiang H. Alpha-synuclein aggregation is involved in the toxicity induced by ferric iron to SK-N-SH neuroblastoma cells. J Neural Transm (Vienna). 2011;118(3):397-406. doi:10.1007/s00702-010-0453-0
  • Lopez A, Lee SE, Wojta K, et al. A152T tau allele causes neurodegeneration that can be ameliorated in a zebrafish model by autophagy induction. Brain. 2017;140(4):1128-1146. doi:10.1093/brain/awx005
  • Weston LJ, Cook ZT, Stackhouse TL, et al. In vivo aggregation of presynaptic alpha-synuclein is not influenced by its phosphorylation at serine-129. Neurobiol Dis. 2021;152. doi:10.1016/j.nbd.2021.105291
  • Prabhudesai S, Sinha S, Attar A, et al. A novel “Molecular Tweezer” inhibitor of ?-Synuclein neurotoxicity in vitro and in vivo. Neurotherapeutics. 2012;9(2):464-476. doi:10.1007/s13311-012-0105-1
  • Mahul-Mellier AL, Burtscher J, Maharjan N, et al. The process of Lewy body formation, rather than simply ?-synuclein fibrillization, is one of the major drivers of neurodegeneration. In: The Proceedings of the National Academy of Sciences (PNAS). Vol 117. ; 2020:4971-4982. doi:10.6084/m9.figshare.11842389.v2
  • Orhan IE. Centella asiatica (L.) urban: From traditional medicine to modern medicine with neuroprotective potential. Evidence-Based Complementary and Alternative Medicine. 2012;2012:1-8. doi:10.1155/2012/946259
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How to Cite This

Gondokesumo, M. E., Muslikh, F. A., Nopitasari, N. P. D., & Putri, P. D. A. (2023). Decreasing alpha-synuclein aggregation on rotenone-induced adult Zebrafish as Parkinson’s Diseases model by ethanol extraction of Keluwih (Artocarpus camansi) leaves. Jurnal Teknologi Laboratorium, 12(2), 53–60. https://doi.org/10.29238/teknolabjournal.v12i2.408

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