Abstract

The skin, as the largest organ in both humans and animals, functions as a protective barrier against various external threats such as sharp objects, temperature fluctuations, chemicals, and physical trauma. Incision wounds are particularly vulnerable to infections, with Staphylococcus aureus being a common causative agent of secondary complications, including prolonged inflammation and delayed healing. This study investigates the efficacy of green betel leaf extract ointment in accelerating the healing process of incision wounds in Wistar rats infected with Staphylococcus aureus. An experimental, post-test-only controlled group design was employed, involving five groups of five rats each. The groups were treated with an ointment base (control), mupirocin ointment (positive control), and green betel leaf extract ointments at concentrations of 10%, 15%, and 20%. Data were analyzed using normality tests, homogeneity tests, and ANOVA. Phytochemical analysis revealed that green betel leaf extract contains alkaloids, terpenoids/steroids, saponins, tannins, and glycosides, all of which are secondary metabolites known for their antimicrobial and wound healing properties. Among the treatment groups, the 15% green betel leaf extract ointment exhibited the most rapid wound closure (average wound healing of 1.44 mm), approaching the effectiveness of mupirocin ointment. Histopathological observations further demonstrated a significant increase in epithelialization and fibroblast proliferation in treated groups compared to controls. In conclusion, green betel leaf extract ointment, particularly at a 15% concentration, shows promising potential as a topical agent for treating Staphylococcus aureus-infected incision wounds in Wistar rats.

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References

• M. Laut et al., "The Effectiveness of Topical Ointment Containing Ethanolic Extract of Acalypha Indica Leaves on Wound Healing," J. Kaji. Vet., vol. 7, no. 1, pp. 1-11, 2019, doi: 10.35508/jkv.v7i1.01.
• C. J. Watung, C. N. Sambou, J. W. Kanter, and R. R. Palandi, "Effectiveness Test of Ethanol Extract of Siamese Pumpkin (Sechium edule (Jacq.) Swartz) Leaves as a Wound Healer in White Rats (Rattus norvegicus)," J. Pharm. Sci., vol. 6, no. 3, pp. 916-922, 2023, doi: 10.36490/journal-jps.com.v6i3.168.
• H. S. E. Rahayu et al., "Ethanolic extract of the natural product of Daun sirih (Piper betle) leaves may impede the effectiveness of the plasma jet contact style for acute wounds," Clin. Plasma Med., vol. 15, no. 18, 2019, doi: 10.1016/j.cpme.2019.100090.
• M. Dena, G. I. Dalimunthe, M. S. Lubis, and Y. P. Rahayu, "Formulation and test of antibacterial activity of aloe vera leaf meat ointment preparation (Aloe vera L.) against Staphylococcus aureus bacteria in festering wound healing," J. Pharm. Sci., no. 1, pp. 268-275, 2023, doi: 10.36490/journal-jps.com.v6i5-si.388.
• G. T. Pamungkas and A. P. Nirwana, "Resistance Test of Staphylococcus aureus to Vancomycin and Ciprofloxacine in Postoperative Patient Samples at 'Jih Solo Hospital,'" Anakes J. Ilm. Anal. Health, vol. 9, no. 1, pp. 42-48, 2023, doi: 10.37012/anakes.v9i1.889.
• Nur Khairunnisa, Lisa Yuniati, A. S. F. Arsal, Hermiaty, and R. F. Syamsu, "Efektifitas Ekstrak Daun Kemangi & Ekstrak Daun Sirih Merah sebagai Anti Mikroba Staphylococcus aureus Penyebab Furunkle," Fakumi Med. J. J. Mhs. Kedokt., vol. 3, no. 2, pp. 106-111, 2023, doi: 10.33096/fmj.v3i2.185.
• D. D. Octora, Samuel Marpaung, and Jhoti Sumitra, "Uji Efektivitas Daya Hambat Kombinasi Ekstrak Etanol Daun Jeruk Purut (Cymbopogon Citratus) Dan Daun Sirih Hijau (Piper Betle L.) Terhadap Bakteri Staphylococcus Aureus," J. Pertanian, vol. 6, no. 1, hal. 41-45, 2023, doi: 10.35451/jfm.v6i1.1860.
• C. A. A. Pramesti, N. Khikmah, and N. Sulistyani, "Uji antibakteri teh hitam dan teh hijau kombucha pada methicillin-resistant Staphylococcus aureus (MRSA) antibacterial test of black tea and green tea combucha against methicilin-resistant Staphylococcus aureus (MRSA)," J. Biol. Udayana, vol. 26, no. 1, pp. 122-131, 2022.
• Nadya, R. Meilina, Keusumawati, and R. N. Za, "Aktivitas Penyembuhan Luka Salep Ekstrak Biji Ketumbar (Coriandrum sativum L.) pada Mencit (Mus musculus L.) yang Terinfeksi Staphylococcus aureus. ) yang Terinfeksi Staphylococcus Aureus Wound Healing Activity of Coriandrum sativum Extract Ointment in Mice Infected with Staphylococcus aureu," J. Heal. Technol. Med, vol. 7, no. 2, pp. 1662-1673, 2021.
• E. Fachrial, J. Lina, H. Harmileni, S. Anggraini, and W. Y. Sihotang, "Hypoglycemic Activity and Safety Assessment of Pediococcus acidilactici Strain DNH16 in Experimental Type 2 Diabetes Mellitus Rats Induced with Streptozotocin," Indones. Biomed. J., vol. 16, no. 1, pp. 31-9, 2024, doi: 10.18585/inabj.v16i1.2781.
• D. N. Aini, D. Ningsih, and G. E. Pramukantoro, "Patch Effectiveness Test of Green Betel Leaf Extract (Piper betle L.) on Rabbit (Oryctolagus cuniculus) Back Wound Healing," J. Sains dan Kesehat, vol. 5, no. 5, pp. 837-849, 2023, doi: 10.25026/jsk.v5i5.1942.
• N. A. Ririn Agustina, Ajeng Dian Pertiwi, "Effectiveness of Green Betel Leaf Ointment (Piper Betle Linn) Against Wounds," Pharm. Tradit. Med., vol. 3, no. 1, pp. 9-9, 2019, [Online]. Available: www.lppm-mfh.com lppm-politeknikmfh@gmail.com
• Z. Afrylyani, J. Rachmawati, and E. Hardi, "The Effect of Kenikir Leaf and Betel Leaf Extract Mixture on Wound Healing in Mice," J-KIP (Journal of Kegur. and Education Science), vol. 3, no. 2, p. 385, 2022, doi: 10.25157/j-kip.v3i2.6302.
• N. Atika, S. Syachruddin, and K. Kusmiyati, "The Effect of Green Betel Leaves (Piper betle L.) Extract on Wounding Healing in Mice (Mus musculus L.)," J. Biol. Trop., vol. 21, no. 1, pp. 103-111, 2021, doi: 10.29303/jbt.v21i1.2282.
• N. Marfu'ah, S. Sha, and Ichawanuddin, "Test of Antibacterial Potential of Staphylococcus Aureus from Ethanol Extract of Green Betel Leaf (Piper betle L.)," vol. 5, no. 2, pp. 1-10, 2021, [Online]. Available: https://ejournal.unida.gontor.ac.id/index.php/pharmasipha/issue/archive
• A. M. Nadhif, M. T. Kamaluddin, and T. Theodorus, "Effectiveness of Dutch Teak Leaf Extract (Guazuma Ulmifolia Lam) on CRP Levels in Wistar Rats Model of Pulmonary Contusion," Sriwij. J. Med., vol. 3, no. 1, pp. 8-14, 2020, doi: 10.32539/sjm.v3i1.135.
• I. Gita, A. Nasution, C. N. Ginting, and L. Chiuman, "Formulation and Effectiveness Testing of Moringa Leaf (Moringa Oleifera) Ethanol Extract Lotion as A Skin Moisturizer Biomedical Science Master's Study Program, Faculty of Medicine, Dentistry and Health Sciences, Universitas Prima Indonesia (Corre," vol. 17, no. 4, pp. 1385-1396, 2024.
• S. E. Davis, S. S. Tulandi, O. S. Datu, F. Sangande, and D. N. Pareta, "Formulation and Testing of Hibiscus Leaf Ethanol Extract Ointment Preparations (Hibiscus rosa-sinensis L.) with Various Ointment Base Variations," Biofarmasetical Trop., vol. 4, no. 2, pp. 66-73, 2021, doi: 10.55724/j.biofar.trop.v4i2.362.
• D. M. Ulfa, S. M. Tulandi, and J. Sulistiyo, “Toxicity Evaluation with Brine Shrimp Lethality Test and Phytochemical Analysis of Some Indonesian Plant Extracts As Potential Anti-Colon Cancer Agents,” Trop. J. Nat. Prod. Res., vol. 8, no. 4, pp. 6864–6867, 2024, doi: 10.26538/tjnpr/v8i4.16.
• Fiska Maya Wardhani, Linda Chiuman, Chrismis Novalinda Ginting, Sahna Ferdinand Ginting, and Ali Napiah Nasution, "Effects of White Turmeric Extract (Curcuma Zedoaria) as a Nephroprotector in Copper-Induced Wistar Male White Rats," J. Indones. Med. Assoc., vol. 69, no. 8, pp. 258-266, 2020, doi: 10.47830/jinma-vol.69.8-2019-186.
• A. Wicaksana and T. Rachman, "Literature Review Article: Identification of Tannin Function Groups in Some Plants with Ftir Instrument," Angew. Chemie Int. Ed. 6(11), 951-952., vol. 3, no. 1, pp. 10-27, 2018, [Online]. Available: https://medium.com/@arifwicaksanaa/understanding-use-case-a7e576e1b6bf
• M. S. Pakaya, M. A. Mustapa, and M. R. Ali, "Antibacterial Potential Test In Agarwood (Gyrinops Versteegii) Stem Extract Towards Escherichia coli and Staphylococcus aureus," vol. 4, no. 1, pp. 443-452, 2022.
• H. C. Gunawan, Y. Yusliana, P. J. Daeli, S. Sarwendah, and L. Chiuman, "Antibacterial Test of Pineapple (Ananas Comosus (L) Merr) Fruit pulp juice against Staphylococcus Aureus bacteria," J. Kedokt. and Kesehat., vol. 15, no. 2, p. 170, 2019, doi: 10.24853/jkk.15.2.170-177.
• J. Felix, T. Suyono, and L. Chiuman, "Effect of Elephant Trunk Leaf Extract on Malondialdehyde and Superoxide Dismutase Levels in Rats with High Activity," Lantanida J., vol. 11, no. 2, p. 147, 2023, doi: 10.22373/lj.v11i2.19116.
• Y. S. Pamungkas, P. Studi, K. Hewan, F. K. Hewan, and U. Brawijaya, "Woe Insision Var. Sapientum (Musa Paradisiaca) On Healing Of Mrsa (Methicillin-Resistant Staphylococcus Aureus)-Infected Wounds In Rats (Rattus Novergicus) Based On Tgf- ? Expression And Number," 2018.
• R. N. Indarala, A. M. Ulfa, and M. P. Angin, "Formulation And Effectiveness Of Early Flower Extract Salep (Clitoria ternatea L.) On Healing Wounds In White Rats (Rattus novergicus)," J. Farm. Malahayati, vol. 5, no. 2, pp. 176-187, 2023, doi: 10.33024/jfm.v5i2.7007.
• N. Cahaya, E. Erfenna, and D. Rahmawanty, "Effect of quercetin gel on the number of fibroblasts and re-epithelialization in the healing process of iia degree burns in male rats," J. Curr. Pharm. Sci., vol. 2, no. 1, pp. 89-96, 2018.
• M. B. Mukti, A. A. G. Jayawardhita, and A. A. G. Arjana, "Effectiveness of Bidara Leaf Extract on Collagen Density in Gingival Incision Wound Healing of Wistar Rats," Bul. Vet. Udayana, no. 158, p. 419, 2022, doi: 10.24843/bulvet.2022.v14.i04.p15.
• N. K. Suwiti, "Histologic Detection of Wound Healing in Skin After Administration of Noni Leaf (Morinda Citrofilia Linn)," Vet. Udayana, vol. 2, no. 1, pp. 1-9, 2010.
• M. V. L. Tumbol, E. V. Rambi, and T. Mamuaya, "Effect of Giving Ethanol Extract of Pakoba Stem Bark (Tricalysia Minahassae) on Histopathological Features of Hepar and Kidney in Male White Rats (Rattus Norvegicus)," Kesmas, vol. 7, no. 5, pp. 1-16, 2018.
• S. Megawati, N. Nur'aini, and D. Kurniasih, "Effectiveness Test Of 96% Etanol Extract Gel Of Short Fruit Leaves (Manihot esculenta Crantz.) On Healing Of Woels In New Zealand White Galurs," J. Farmagazine, vol. 7, no. 1, p. 1, 2020, doi: 10.47653/farm.v7i1.159.
• D. sartika MS, W. Sinrang, R. Yulianty, and S. Sakinah, "Effectiveness of Betel Leaf Extract Ointment (Piper betle Linn.) on Epithelialization in Wistar Rats with Acute Injury Model," J. Penelit. Health. Suara Forikes, vol. 11, no. 4, pp. 121-121, 2020, [Online]. Available: http://forikes-ejournal.com/index.php/SF
• A. Akbar, A. B. Gani, M. I. Wahab, E. Syahril, and B. E. Hasbi, "Comparison of the Use of Betel Leaf (Piper betle L) and Povidone Iodine on Wound Healing," J. Mhs. Kedokt., vol. 2, no. 5, pp. 359-367, 2022.

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