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Histology

Bidara Leaf Extract (Ziziphus mauritiana L.): A Natural Approach to Enhancing Pancreatic Function and Lowering Blood Sugar in Male Wistar Rats

Musdayani Nasution Universitas Prima Indonesia, Indonesia Correspondence nmusdayani@gmail.com
,
Ali Napiah Nasution Department of Biomedical Sciences, Faculty of Medicine, Dentistry, and Health Sciences, Universitas Prima Indonesia, Medan, Indonesia , Indonesia Correspondence alinapiahnasution@unprimdn.ac.id
,
Maya Sari Mutia Department of Biomedical Sciences, Faculty of Medicine, Dentistry, and Health Sciences, Universitas Prima Indonesia, Medan, Indonesia , Indonesia Correspondence alinapiahnasution@unprimdn.ac.id
Pages 44-52
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Abstract

Diabetes mellitus is a metabolic disorder characterized by insufficient insulin production, leading to elevated blood glucose levels. When pancreatic beta cells produce inadequate insulin to compensate for increased insulin resistance, chronic hyperglycaemia ensues. This study investigates the effect of Bidara leaf extract (Ziziphus mauritiana) on the pancreatic function of male Wistar rats and its efficacy in reducing blood sugar levels. The experimental design was a post-test only controlled group study. Six groups were included: a control group receiving 1% Na CMC and five treatment groups receiving Bidara leaf ethanol extract at doses of 50 mg/kg, 100 mg/kg, 300 mg/kg, and 500 mg/kg, with each group consisting of four rats. Data analysis involved normality tests, homogeneity tests, and ANOVA. Results indicated that the p-value for each group was less than 0.05. Specifically, the 50 mg/kg dose of Bidara leaf extract significantly reduced blood sugar levels (p = 0.02). The 100 mg/kg dose showed a p-value of 0.14, indicating effectiveness in lowering blood sugar levels, though less pronounced. Similarly, the 300 mg/kg dose (p = 0.16) and the 500 mg/kg dose (p = 0.04) demonstrated significant hypoglycaemic effects. In conclusion, Bidara leaf extract has a dose-dependent effect on lowering blood sugar levels in male Wistar rats, with significant results observed at varying concentrations. Further research is recommended to explore the mechanisms involved and potential therapeutic applications in humans.

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References

  • Petrus P, Suwarni S, Haerani WO. Hubungan Asupan Karbohidrat dan Aktivitas Fisik dengan Kadar Glukosa Darah Puasa Penderita Diabetes Melitus Tipe 2. Heal Inf J Penelit. 2023;15(3):1047.doi:10.36990/hijp.v15i3.1047.
  • Djrami J, Niwele A, Polpoke N. Uji Farmakologi Ekstrak Etanol 70% Daun Kersen (Muntigua Calabula L) Terhadap Penurunan Kadar Glukosa Darah Pada Mencit (Mus Musculus. J Ris Rumpun Ilmu Kesehat. 2023;1(1):133-149,. doi:10.55606/jurrikes.v1i1.897.
  • Yuliastri WO. Uji Efek Ekstrak Bawang Hitam (Allium sativum) terhadap Penurunan Kadar Glikosa Darah pada Tikus Putih (Rattus novergicus L) dengan Metode Tes Toleransi Glukosa Oral (TTGO) Test Effect Of Black Garlic (Allium sativum) Extract Againts Decreased Blood Gluco. 53 Pharm. 2020;01:53-63,.
  • Haidir YP, Saputri GAR, Hermawan D. Uji Efektivitas Kombinasi Bawang Dayak (Eleutherine Palmifolia (L.) Merr) Dan Daun Insulin (Tithonia Diversifolia) Terhadap Penurunan Kadar Glukosa Darah Pada Tikus Putih (Rattus Norvegicus) Diinduksi Na2Edta. J Farm Malahayati. 2022;5(1):86-97,. doi:10.33024/jfm.v5i1.7004.
  • Syarifuddin S, Marpaung RF, Hotria P. Penggunaan Obat Antidiabetes Pada Pasien Rawat Inap Di Rumah Sakit Harapan Pematangsiantar. Klin Sains J Anal Kesehat. 2021;9(1):23-35,. doi:10.36341/klinikal_sains.v9i1.1869.
  • Sutriyawan A, Yusuff AA, Fardhoni HA, Fibrianti. Retrospective study of lifestyle and incidence of Type 2 Diabetes Mellitus at Arcamanik Health Center Bandung City. Issues Biol Sci Pharm Res. 2022;10(2):19-23,. doi:10.15739/ibspr.22.004.
  • Avila DEM. Prevalensi dan Karakteristik Pasien Retinopati Diabetik pada Pasien Prolanis di RSI Gondanglegi Tahun 2021. J Ilm Indones. 2022;7(8.5.2017):2003-2005,.
  • Sun H. IDF Diabetes Atlas: Global, regional and country-level diabetes prevalence estimates for 2021 and projections for 2045. Diabetes Res Clin Pract. 2022;183:1-23,. doi:10.1016/j.diabres.2021.109119.
  • Ulfa AM, Junaida R. Identifikasi Kandungan Senyawa Kimia Dan Analisis Proksimat Terhadap Ekstrak Etanol Daun Bidara Arab (Ziziphus Mauritiana L. J Heal Educ Sci Technol. 2023;6(2):125-132,. doi:10.25139/htc.v6i2.6775.
  • Lestari G, Samudera AG, Safira AR. Formulasi Sediaan Krim Tipe M/A Ekstrak Daun Bidara Arab (Ziziphus Mauritiana Lam) Sebagai Antiinflamasi Terhadap Mencit Putih Jantan (Mus musculus L. J Sains Kesehat. 2022;29(1):9-17,. doi:10.37638/jsk.29.1.9-17.
  • Putri A, Nofita N, Ulfa AM. Perbandingan Aktivitas Antioksidan Ekstrak Daun Bidara (Ziziphus Spina-Christi L.) Dengan Teknik Ekstraksi Perkolasi Dan INFUSA. J Ilmu Kedokt dan Kesehat. 2023;9(4):1178-1189,. doi:10.33024/jikk.v9i4.5635.
  • Nofita N, Rosidah DNU, Yusuf M. Perbandingan Aktivitas Antioksidan Ekstrak Daun Bidara (Ziziphus Spina-Christi L.) Menggunakan Pelarut Etanol Dan N-Heksana. J Ilmu Kedokt dan Kesehat. 2022;9(3):924-933,. doi:10.33024/jikk.v9i3.5562.
  • Haeria H, Dg.Pine AT. Penentuan Kadar Flavonoid Total dan Aktivitas Antioksidan Ekstrak Etanol Daun Bidara (Ziziphus spina-christi L.) Haeria. J Pharm Med Sci. 2016;1(2):57-61,.
  • Wardhani FM, Chiuman L, Ginting CN, Ginting SF, Nasution AN. Efek Ekstrak Kunyit Putih (Curcuma Zedoaria) Sebagai Nefroprotektor Pada Tikus Putih Jantan Galur Wistar yang Diinduksi Tembaga. J Indones Med Assoc. 2020;69(8):258-266,. doi:10.47830/jinma-vol.69.8-2019-186.
  • Tambunan GCA, Girsang E, Nasution AN. Pengaruh Pemberian Gel Ekstrak Daun Pegagan (Cantella asiatica) sebagai Peningkat Neovaskularisasi, Fibroblast dan Epitalisasi dalam Penyembuhan Luka Tikus Jantan. Hijp Heal Inf J Penelit. 2023;15:957,.
  • Panjaitan J, Girsang E, Chiuman L. Effectiveness of temulawak (Curcuma xanthorrhiza) ointments as wound healing agents in wistar rats. J Teknol Lab. 2022;11(2):95-104,. doi:10.29238/teknolabjournal.v11i2.376.
  • Studi P. Profl Hematologi Tikus Putih (Rattus Norvegicus) yang Diberi Salep Simplisia Daun Kembang Sepatu (Hibiscus Rosa-Sinensis L.) setelah Dipapar Sinar Ultraviolet. J Sain Vet. 2023;41:226-238,.
  • Velanie CS, Diarti MW, Pauzi I, Jurusan A, Kesehatan K, Mataram I. Pemberian Tepung Beras Merah (Oryza Nivara) Terhadap Penurunan Kadar Glukosa Darah Pada Hewan Coba Tikus Putih (Rattus Norvegicus) Galur Wistar. J Anal Med Bio Sains. 2017;4(2):81-86,.
  • Pratama IH, Chaniago SSY, Pulungan IY, Siagian AS, Silalahi LN. Gambaran Histopatologi Dan Uji Efektivitas Biji Ketumbar (Coriandrum Sativum L) Pada Ginjal Tikus Wistar (Rattus Norvegicus) Yang Diinduksi Aloksan. J Kesehat Tambusai. 2023;4(4):6883-6888,. doi:10.31004/jkt.v4i4.22574.
  • Rosmarwati E, Ellistasari EY, Kusumawardani A. Gambaran klinis dan histopatologi kulit tikus Sprague-Dawley pada kelompok tikus muda, tua, dan tikus model tua induksi D-galaktosa. Herb-Medicine J Terbit Berk Ilm Herbal, Kedokt dan Kesehat. 2023;5(3):1,. doi:10.30595/hmj.v5i3.14894.
  • Ismawati I, Romus I, Utami EA. Efek Inhibitor Proteasom terhadap Histopatologi Arteri Koronaria pada Tikus Model Aterosklerosis. J Kedokt dan Kesehat. 2020;16(2):135,. doi:10.24853/jkk.16.2.135-142.
  • Nadhif AM, Kamaluddin MT, Theodorus T. Efektivitas Pemberian Ekstrak Daun Jati Belanda (Guazuma Ulmifolia Lam) terhadap Kadar CRP pada Tikus Wistar Model Kontusio Pulmonum. Sriwij J Med. 2020;3(1):8-14,. doi:10.32539/sjm.v3i1.135.
  • Putri Mambang R D Elysa, Suryanto D. Aktivitas Antibakteri Ekstrak Tempe Terhadap Bakteri Bacillus Subtilis Dan Staphylococcus Aureus. J Teknol Dan Ind Pangan. 2014;25(1):115-118,. doi:10.6066/jtip.2014.25.1.115.
  • Maharani ADK, Santoso P, Udayani NNW. Efek Ekstrak Etanol Buah Dewandaru (Eugenia uniflora L) terhadap Penurunan Kadar Glukosa Darah dan Perbaikan Sel Beta Pankreas pada Mencit yang Diinduksi Aloksan. Usadha. 2023;2(2):39-44,. doi:10.36733/usadha.v2i3.7384.
  • Dewi IP, Dharma S, Marlina M. Pengaruh Pemberian Fibroblast Growth Factor (FGF) dari Telur Ayam Terfertilisasi Terhadap Kadar Glukosa Darah Mencit Hiperglikemia. J Sains Farm Klin. 2016;3(1):1,. doi:10.29208/jsfk.2016.3.1.86.
  • Monikasari M, Widyastiti NS, Mahati E, Syauqy A, Al-Baarri AN. Pengaruh pemberian ekstrak bekatul beras hitam (Oryza sativa L. indica) terhadap kadar MDA, SOD dan trigliserida pada tikus diabetes mellitus tipe 2. AcTion Aceh Nutr J. 2023;8(1):129,. doi:10.30867/action.v8i1.731.
  • Noviati TD, Renowening Y, Maarif MZ, Mahmudah H, Ridha A. Pengaruh Ubi Jalar Ungu terhadap Diabetes Mellitus Tipe 2. J Promot Prev. 2023;6(2):344-351,.
  • Anjani EP, Oktarlina RZ, Morfi CW. Zat Antosianin pada Ubi Jalar Ungu terhadap Diabetes Melitus The Substances Anthocyanins in Purple Sweet Potato Against Diabetes Mellitus. Majority. 2018;7(2):257-262,.
  • Hutahean H. Uji antidiabetes kombinasi ekstrak daun insulin (Tithonia diversifolia (Hems.) A.Gray) dan daun ubi jalar (Ipomoea batatas (L.) Lam) ungu pada tikus jantan akibat induksi streptozotocin. Juornal Econ Strateg. 2020;1(1):1-10,.
  • Siregar M. Berbagai Manfaat Daun Bidara (Ziziphus mauritiana Lamk) Bagi Kesehatan di Indonesia?: Meta Analisis. J Pandu Husada. 2020;1(2):75,. doi:10.30596/jph.v1i2.4415.
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How to Cite This

Nasution, M., Nasution, A. N., & Mutia, M. S. (2024). Bidara Leaf Extract (Ziziphus mauritiana L.): A Natural Approach to Enhancing Pancreatic Function and Lowering Blood Sugar in Male Wistar Rats. Jurnal Teknologi Laboratorium, 13(1), 44–52. https://doi.org/10.29238/teknolabjournal.v13i1.476

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