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Biotechnology

Phytochemical Profile and Antibacterial Activity of Fermented Kombucha Black Tea (Camellia sinensis) Extract Against Acne-associated Bacteria

Larastika Yovianda Department of Biomedical Sciences, Faculty of Medicine, Dentistry, and Health Sciences, Universitas Prima Indonesia, Medan, Indonesia, Indonesia Correspondence larastikayovianda@yahoo.com
,
Merisca Gianthra Ryosa Department of Biomedical Sciences, Faculty of Medicine, Dentistry, and Health Sciences, Universitas Prima Indonesia, Medan, Indonesia, Indonesia Correspondence aira10102015@gmail.com
,
Ully Chairunisa Department of Pharmacy, College of Pharmaceutical Sciences, Padang, West Sumatra, Indonesia, Indonesia Correspondence refiikhtiari@unprimdn.ac.id
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Erny Tandanu Department of Medical Education, Faculty of Medicine, Dentistry, and Health Sciences, Universitas Prima Indonesia, Medan, Indonesia Department of Clinical , Indonesia Correspondence ernytandanu@unprimdn.ac.id
,
Refi Ikhtiari Department of Biomedical Sciences, Faculty of Medicine, Dentistry, and Health Sciences, Universitas Prima Indonesia, Medan, Indonesia, Indonesia Correspondence refiikhtiari@unprimdn.ac.id
Pages 109-117
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Abstract

This study aimed to investigate the phytochemical profile and antibacterial efficacy of Kombucha black tea extract fermented for different durations (12, 18, and 20 days) against acne-causing bacteria. The research included qualitative phytochemical screening of alkaloid, flavonoid, saponin, steroid, and triterpenoid group identifications using specific assays. The study involved qualitative phytochemical screening and quantitative determination of total flavonoid content, expressed in milligrams of Quercetin Equivalents per gram of extract (mg QE/g). Antibacterial activity was evaluated using the disk diffusion method against Cutibacterium acnes, Staphylococcus aureus, and MRSA. Results showed that the extract is rich in flavonoids, phenols, saponins, and triterpenoids. The total flavonoid content varied significantly with fermentation time: 59.70 mg QE/g (Day 12), 80.28 mg QE/g (Day 18), and 71.95 mg QE/g (Day 20). The Day 18 extract demonstrated the strongest antibacterial inhibition against C. acnes (p < 0.05). In conclusion, optimized fermented Kombucha black tea extract exhibits significant antibacterial potential specifically targeting C. acnes, serving as a promising natural active ingredient for anti-acne therapy.

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How to Cite This

Yovianda, L., Merisca Gianthra Ryosa, Ully Chairunisa, Erny Tandanu, & Ikhtiari, R. (2026). Phytochemical Profile and Antibacterial Activity of Fermented Kombucha Black Tea (Camellia sinensis) Extract Against Acne-associated Bacteria . Jurnal Teknologi Laboratorium, 15(1), 109–117. https://doi.org/10.29238/teknolabjournal.v15i1.509

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All data generated during this study are included in this published article [and its supplementary information files].