Abstract

This study evaluated the effectiveness of a 15% cork fish extract hydrogel in promoting subcutaneous wound healing in male Wistar rats. The objectives were to examine its impact on wound healing, eosinophil counts, allergic reactions, and body weight changes. A total of 30 male Wistar rats aged 6–8 weeks (150–200 g) were randomly assigned to six groups: 15%, 10%, and 5% cork fish hydrogel treatments, positive control (Bioplacenton), negative control, and normal control. Statistical analysis using the Kruskal-Wallis test revealed significant effects of hydrogel treatments on eosinophil counts (p = 0.032), while ANOVA showed significant differences in wound diameter reduction (p = 0.011). Spearman correlation analysis identified a moderate positive correlation between eosinophil count and wound shrinkage (r = 0.420, p = 0.037). No adverse effects or allergic reactions were observed in any treatment group, and body weight remained unaffected (p = 0.365). These findings indicate that the 15% cork fish hydrogel effectively enhances wound healing in rats, demonstrating its potential as a safe and effective therapeutic agent for subcutaneous wound management.

There is no Figure or data content available for this article

References

• 1. Grace Noviyanthi Sinambela, Tandanu E, Ikhtiari R. The wound healing effect of Morinda citrifolia leaf extract and biomolecular analysis on inflammation and proliferation stages in Wistar rats. Jurnal Teknologi Laboratorium. 2022;11(2). doi:10.29238/teknolabjournal.v11i2.369
• 2. ARFANI A, RAIF A, GINTING CN, IKHTIARI R. Evaluation of wound healing potential of a sea cucumber (Actinopyga mauritiana) extract in mice (Mus musculus). Jurnal Natural. 2021;21(3). doi:10.24815/jn.v21i3.19953
• 3. Hayati W, Raif MA, Ginting CN, Ikhtiari R. Antioxidant and Wound Healing Potential of Persea Americana Mill. Leaves extract. In: AIMS 2021 - International Conference on Artificial Intelligence and Mechatronics Systems. ; 2021. doi:10.1109/AIMS52415.2021.9466076
• 4. Heryani DW, Wardhani FM, Yensuari, Putri NN, Nardi L, Ikhtiari R. Topical Application of Paraboea leuserensis on Excision Wound with Angiogenesis and Vascular Endothelial Growth Factor Analysis. Hayati. 2024;31(2). doi:10.4308/hjb.31.2.300-316
• 5. Aidah N, Nyoman Ehrich Lister I, Ginting CN, Ikhtiari R. Evaluation of Honey Effectivity on Burned-Wound Contraction in Rattus Norvegicus. In: InHeNce 2021 - 2021 IEEE International Conference on Health, Instrumentation and Measurement, and Natural Sciences. ; 2021. doi:10.1109/InHeNce52833.2021.9537239
• 6. Surya KE, Khu A, Raif A, Ikhtiari R. Papaya Latex for Healing the Second Degree of Burn Wound in Male Mice. In: InHeNce 2021 - 2021 IEEE International Conference on Health, Instrumentation and Measurement, and Natural Sciences. ; 2021. doi:10.1109/InHeNce52833.2021.9537269
• 7. Han G, Ceilley R. Chronic Wound Healing: A Review of Current Management and Treatments. Adv Ther. 2017;34(3). doi:10.1007/s12325-017-0478-y
• 8. Murphy PS, Evans GRD. Advances in Wound Healing: A Review of Current Wound Healing Products. Plast Surg Int. 2012;2012. doi:10.1155/2012/190436
• 9. Hao R, Cui Z, Zhang X, et al. Rational Design and Preparation of Functional Hydrogels for Skin Wound Healing. Front Chem. 2022;9. doi:10.3389/fchem.2021.839055
• 10. Liang Y, He J, Guo B. Functional Hydrogels as Wound Dressing to Enhance Wound Healing. ACS Nano. 2021;15(8). doi:10.1021/acsnano.1c04206
• 11. Zhang S, Ge G, Qin Y, et al. Recent advances in responsive hydrogels for diabetic wound healing. Mater Today Bio. 2023;18. doi:10.1016/j.mtbio.2022.100508
• 12. Fan F, Saha S, Hanjaya-Putra D. Biomimetic Hydrogels to Promote Wound Healing. Front Bioeng Biotechnol. 2021;9. doi:10.3389/fbioe.2021.718377
• 13. Asadi N, Pazoki-Toroudi H, Del Bakhshayesh AR, Akbarzadeh A, Davaran S, Annabi N. Multifunctional hydrogels for wound healing: Special focus on biomacromolecular based hydrogels. Int J Biol Macromol. 2021;170. doi:10.1016/j.ijbiomac.2020.12.202
• 14. Chelu M, Musuc AM, Popa M, Calderon Moreno J. Aloe vera-Based Hydrogels for Wound Healing: Properties and Therapeutic Effects. Gels. 2023;9(7). doi:10.3390/gels9070539
• 15. Shrestha C, Zhao L, Chen K, He H, Mo Z. Enhanced healing of diabetic wounds by subcutaneous administration of human umbilical cord derived stem cells and their conditioned media. Int J Endocrinol. 2013;2013. doi:10.1155/2013/592454
• 16. Jiang D, Rinkevich Y. Furnishing wound repair by the subcutaneous fascia. Int J Mol Sci. 2021;22(16). doi:10.3390/ijms22169006
• 17. Janssen N, Laven IEWG, Daemen JHT, Hulsewé KWE, Vissers YLJ, de Loos ER. Negative pressure wound therapy for massive subcutaneous emphysema: a systematic review and case series. J Thorac Dis. 2022;14(1). doi:10.21037/jtd-21-1483
• 18. Ferreira MC, Tuma P, Carvalho VF, Kamamoto F. Complex wounds. Clinics. 2006;61(6). doi:10.1590/S1807-59322006000600014
• 19. Manzoor B, Heywood N, Sharma A. Review of Subcutaneous Wound Drainage in Reducing Surgical Site Infections after Laparotomy. Surg Res Pract. 2015;2015. doi:10.1155/2015/715803
• 20. Vaidyanathan L. Growth factors in wound healing ? a review. Biomedical and Pharmacology Journal. 2021;14(3). doi:10.13005/bpj/2249
• 21. Simões D, Miguel SP, Ribeiro MP, Coutinho P, Mendonça AG, Correia IJ. Recent advances on antimicrobial wound dressing: A review. European Journal of Pharmaceutics and Biopharmaceutics. 2018;127. doi:10.1016/j.ejpb.2018.02.022
• 22. Sarabahi S. Recent advances in topical wound care. Indian Journal of Plastic Surgery. 2012;45(2). doi:10.4103/0970-0358.101321
• 23. Yao Y, Zhang A, Yuan C, Chen X, Liu Y. Recent trends on burn wound care: Hydrogel dressings and scaffolds. Biomater Sci. 2021;9(13). doi:10.1039/d1bm00411e
• 24. Le Pillouer-Prost A, Sales-Ausias N. Dressings’ classes. Nouvelles Dermatologiques. 2008;27(7 PART 2).
• 25. Sharma A, Sharma D, Zhao F. Updates on Recent Clinical Assessment of Commercial Chronic Wound Care Products. Adv Healthc Mater. 2023;12(25). doi:10.1002/adhm.202300556
• 26. Wardhani FM, Chiuman L, Novalinda Ginting C, Ferdinand Ginting S, Napiah Nasution A. Efek Ekstrak Kunyit Putih (Curcuma Zedoaria) Sebagai Nefroprotektor Pada Tikus Putih Jantan Galur Wistar yang Diinduksi Tembaga. Journal of the Indonesian Medical Association. 2019;69(8).
• 27. Masson-Meyers DS, Andrade TAM, Caetano GF, et al. Experimental models and methods for cutaneous wound healing assessment. Int J Exp Pathol. 2020;101(1-2). doi:10.1111/iep.12346
• 28. Ainge Rasbina Br Saragih, Fiska Maya Wardhani, Tandanu E, Rico Alexander. Acute Toxicity Testing of White Turmeric Extract (Curcuma zedoaria) on Histopathological Imaging of the Lungs. Archives of The Medicine and Case Reports. 2021;2(4). doi:10.37275/amcr.v2i4.125
• 29. Fiska Maya Wardhani, Linda Chiuman, Chrismis Novalinda Ginting, Sahna Ferdinand Ginting, Ali Napiah Nasution. Efek Ekstrak Kunyit Putih (Curcuma Zedoaria) Sebagai Nefroprotektor Pada Tikus Putih Jantan Galur Wistar yang Diinduksi Tembaga. Journal Of The Indonesian Medical Association. 2020;69(8). doi:10.47830/jinma-vol.69.8-2019-186
• 30. Gani JO, Wardhani FM, Tandanu E. UJI TOKSISITAS AKUT EKSTRAK KUNYIT PUTIH (Curcuma zedoaria) PADA GINJAL TIKUS WISTAR JANTAN. Majalah Kesehatan. 2021;8(4). doi:10.21776/ub.majalahkesehatan.2021.008.04.2
• 31. Wardhani FM, Ong GF, Virgoh L, Lubis A, Nasution MH. UJI TOKSISITAS AKUT EKSTRAK KUNYIT PUTIH TERHADAP KADAR GULA DARAH DAN KOLESTEROL. Jurnal Kedokteran dan Kesehatan?: Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya. 2022;9(3). doi:10.32539/jkk.v9i3.19028
• 32. Qin Q, Haba D, Takizawa C, et al. A method for harvesting viable cells from wound dressings. Exp Dermatol. 2023;32(9). doi:10.1111/exd.14857
• 33. Ali A, Garg P, Goyal R, et al. A novel herbal hydrogel formulation of moringa oleifera for wound healing. Plants. 2021;10(1). doi:10.3390/plants10010025
• 34. Coden ME, Berdnikovs S. Eosinophils in wound healing and epithelial remodeling: Is coagulation a missing link? J Leukoc Biol. 2020;108(1). doi:10.1002/JLB.3MR0120-390R
• 35. Todd R, Donoff BR, Chiang T, et al. The eosinophil as a cellular source of transforming growth factor alpha in healing cutaneous wounds. American Journal of Pathology. 1991;138(6).
• 36. Zhang T, Day JH, Su X, et al. Investigating fibroblast-induced collagen gel contraction using a dynamic microscale platform. Front Bioeng Biotechnol. 2019;7(AUG). doi:10.3389/fbioe.2019.00196
• 37. Li W, Zheng Y, Pang W, Lai P. Bio-inspired adhesive hydrogel for wound healing. Biomedical Technology. 2023;1. doi:10.1016/j.bmt.2022.11.009
• 38. Yang D, Chen H, Wei H, Liu A, Wei DX, Chen J. Hydrogel wound dressings containing bioactive compounds originated from traditional Chinese herbs: A review. Smart Mater Med. 2024;5(1). doi:10.1016/j.smaim.2023.10.004
• 39. Surowiecka A, Stru?yna J, Winiarska A, Korzeniowski T. Hydrogels in Burn Wound Management—A Review. Gels. 2022;8(2). doi:10.3390/gels8020122
• 40. Zhang L, Yin H, Lei X, et al. A Systematic Review and Meta-Analysis of Clinical Effectiveness and Safety of Hydrogel Dressings in the Management of Skin Wounds. Front Bioeng Biotechnol. 2019;7. doi:10.3389/fbioe.2019.00342
• 41. Yosefa SR, Tandanu E, Leslie W, et al. In Vitro and In Vivo Antidiarrheal Activity of Dragon Fruit Peels Methanolic Extract. Majalah Obat Tradisional. 2023;28(2). doi:10.22146/mot.81216
• 42. Zhu M, Li W, Dong X, et al. In vivo engineered extracellular matrix scaffolds with instructive niches for oriented tissue regeneration. Nat Commun. 2019;10(1). doi:10.1038/s41467-019-12545-3
• 43. Lombardi F, Palumbo P, Augello FR, Cifone MG, Cinque B, Giuliani M. Secretome of adipose tissue-derived stem cells (ASCs) as a novel trend in chronic non-healing wounds: An overview of experimental in vitro and in vivo studies and methodological variables. Int J Mol Sci. 2019;20(15). doi:10.3390/ijms20153721
• 44. Roodbari N, Sotoudeh A, Jahanshahi A, Takhtfooladi MA. Healing effect of Adiantumcapillus veneris on surgical wound in rat. Res Opin Anim Vet Sci. 2012;2(12).
• 45. Andrie M, Taurina W. Ointment formulation of snakehead fish (Channa striata) Extract with variations of CMC-Na and carbopol. Pharmaciana. 2021;11(1). doi:10.12928/pharmaciana.v11i1.18385
• 46. Wilkinson HN, Hardman MJ. Wound healing: Cellular mechanisms and pathological outcomes. Advances in Surgical and Medical Specialties. Published online 2023:341-370. doi:10.1098

How to Cite This

Article Metrics

Download Statistics

Downloads

Download data is not yet available.

Other Statistics

Copyright and Permissions

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Publishing your paper with Jurnal Teknologi Laboratorium (JTL) means that the author or authors retain the copyright in the paper. JTL granted an exclusive reuse license by the author(s), but the author(s) are able to put the paper onto a website, distribute it to colleagues, give it to students, use it in your thesis etc, even commercially. The author(s) can reuse the figures and tables and other information contained in their paper published by JTL in future papers or work without having to ask anyone for permission, provided that the figures, tables or other information that is included in the new paper or work properly references the published paper as the source of the figures, tables or other information, and the new paper or work is not direct at private monetary gain or commercial advantage.

JTL journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. This journal is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License. This license lets others remix, transform, and build upon the material for any purpose, even commercially.

JTL journal Open Access articles are distributed under this Creative Commons Attribution-ShareAlike 4.0 International License (CC BY-SA). Articles can be read and shared for All purposes under the following conditions:

• BY: You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
• SA:  If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.

Data Availability