126
Abstract Views
33
PDF Download
Immunology

Topical pomegranate (Punica granatum) extract cream accelerates wound healing by modulating VEGF and IL-10 expression in rats

Citra Dewi Hartati Department of Biomedical Sciences, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, Indonesia, Indonesia Correspondence citradewihartati0904@gmail.com
,
Titiek Sumarawati Department of Biomedical Sciences, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, Indonesia, Indonesia Correspondence sumarawati@unissula.ac.id
,
Agung Putra Department of Biomedical Sciences, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, Indonesia, Indonesia Correspondence dr.agungptr@gmail.com
Pages 206-220
Browse this journal

Abstract

Wound healing is a complex biological process aimed at restoring the integrity and function of damaged tissues. The demand for effective and aesthetically satisfying wound repair has driven interest in natural therapeutic alternatives to synthetic drugs. Punica granatum (pomegranate) has been reported to possess significant wound healing properties. This study aimed to evaluate the effect of topical pomegranate extract cream on the expression of vascular endothelial growth factor (VEGF) and interleukin-10 (IL-10) in an excision wound model in rats. A post-test-only control group design was used with 48 male Wistar rats divided into 12 treatment groups. VEGF and IL-10 levels were measured on day 3 and day 7 after treatment. Statistical analysis using One-Way ANOVA and Tamhane’s post hoc test showed significant differences in VEGF and IL-10 expression across treatment groups (p = 0.000), with the highest levels observed in the 20% pomegranate cream group on both days (p < 0.05). These findings indicate that topical pomegranate extract cream enhances wound healing by increasing VEGF and IL-10 expression, particularly at the 20% concentration.

There is no Figure or data content available for this article

References

  • 1. Nagoba B, Davane M. Studies on wound healing potential of topical herbal formulations- do we need to strengthen study protocol? J Ayurveda Integr Med.Elsevier B.V. 2019;10(4):316-318. doi:10.1016/j.jaim.2019.09.002
  • 2. Jourdan M, Madfes DC, Lima E, Tian Y, Seité S. Skin care management for medical and aesthetic procedures to prevent scarring. Clin Cosmet Investig Dermatol.Dove Medical Press Ltd. 2019;12:799-804. doi:10.2147/CCID.S218134
  • 3. Wise LM, Stuart GS, Real NC, Fleming SB, Mercer AA. Orf virus IL-10 accelerates wound healing while limiting inflammation and scarring. Wound Repair and Regeneration. 2014;22(3):356-367. doi:10.1111/wrr.12169
  • 4. Birkenhauer E, Neethirajan S. A double-edged sword: The role of VEGF in wound repair and chemoattraction of opportunist pathogens. Int J Mol Sci. 2015;16(4):7159-7172. doi:10.3390/ijms16047159
  • 5. Shams F, Moravvej H, Hosseinzadeh S, et al. Overexpression of VEGF in dermal fibroblast cells accelerates the angiogenesis and wound healing function: in vitro and in vivo studies. Sci Rep. 2022;12(1). doi:10.1038/s41598-022-23304-8
  • 6. Bigliardi PL, Alsagoff SAL, El-Kafrawi HY, Pyon JK, Wa CTC, Villa MA. Povidone iodine in wound healing: A review of current concepts and practices. International Journal of Surgery.Elsevier Ltd. 2017;44:260-268. doi:10.1016/j.ijsu.2017.06.073
  • 7. Shedoeva A, Leavesley D, Upton Z, Fan C. Wound healing and the use of medicinal plants. Evidence-based Complementary and Alternative Medicine.Hindawi Limited. 2019;2019. doi:10.1155/2019/2684108
  • 8. Akbarnejad F. Dermatology Benefits of Punica Granatum: A Review of the Potential Benefits of Punica Granatum in Skin Disorders. Asian Journal of Green Chemistry.Sami Publishing Company. 2023;7(3):208-222. doi:10.22034/ajgc.2023.388077.1388
  • 9. Sen CK. Human Wounds and Its Burden: An Updated Compendium of Estimates. Adv Wound Care (New Rochelle).Mary Ann Liebert Inc. 2019;8(2):39-48. doi:10.1089/wound.2019.0946
  • 10. Pal M, Saxena R, Kanya R, et al. A Detailed Analysis of Herbal Cosmetics. NeuroQuantology.?;20(15):6367. Published online 2022. doi:10.48047/NQ.2022.20.15.NQ88637
  • 11. Essa MM, Subash S, Akbar M, Al-Adawi S, Guillemin GJ. Long-Term dietary supplementation of pomegranates, figs and dates alleviate neuroinflammation in a transgenic mouse model of alzheimer’s disease. PLoS One. 2015;10(3). doi:10.1371/JOURNAL.PONE.0120964,
  • 12. Lukiswanto BS, Miranti A, Sudjarwo SA, Primarizky H, Yuniarti WM. Evaluation of wound healing potential of pomegranate (Punica granatum) whole fruit extract on skin burn wound in rats (Rattus norvegicus). J Adv Vet Anim Res. 2019;6(2):202-207. doi:10.5455/JAVAR.2019.F333,
  • 13. Medellín-Luna MF, Castañeda-Delgado JE, Martínez-Balderas VY, Cervantes-Villagrana AR. Medicinal Plant Extracts and Their Use As Wound Closure Inducing Agents. J Med Food.Mary Ann Liebert Inc. 2019;22(5):435-443. doi:10.1089/jmf.2018.0145
  • 14. Kumar T, Malik R, Zahrah Maqbool S. Herbal plant with potensial of wound healing activity: a review article. www.wjpps.com ?. 2015;12:333. doi:10.20959/wjpps20233-24260
  • 15. Nirwana I, Rachmadi P, Rianti D. Potential of pomegranate fruit extract (Punica granatum Linn.) to increase vascular endothelial growth factor and platelet-derived growth factor expressions on the post-tooth extraction wound of Cavia cobaya. Vet World. 2017;10(8):999-1003. doi:10.14202/VETWORLD.2017.999-1003,
  • 16. Rakhshandeh H, Baradaran Rahimi V, Habibi Z, Sirousi Z, Askari VR. Punica granatum seed oil detracts peritoneal adhesion: Perusing antioxidant, anti-inflammatory, antifibrotic, and antiangiogenic impacts. Physiol Rep. 2022;10(24). doi:10.14814/PHY2.15545,
  • 17. Mehdi A, Lamiae B, Samira B, et al. Pomegranate (Punica granatum L.) Attenuates Neuroinflammation Involved in Neurodegenerative Diseases. Foods. 2022;11(17). doi:10.3390/FOODS11172570,
  • 18. Sari N, Samsul E, Narsa AC. Pengaruh Trietanolamin pada Basis Krim Minyak dalam Air yang Berbahan Dasar Asam Stearat dan Setil Alkohol. Proceeding of Mulawarman Pharmaceuticals Conferences. 2021;14:70-75. doi:10.25026/mpc.v14i1.573
  • 19. Shinde VM, Shende A, Mahadik K. Evaluation Of Antioxidant And Wound Healing Potential Of Pomegranate Peel Gel Formulation. International Journal of Pharmacognosy. 2020;7(1):23-28. doi:10.13040/IJPSR.0975-8232.IJP.7(1).23-28
  • 20. Nasiri E, Hosseinimehr SJ, Akbari J, Azadbakht M, Azizi S. The Effects of Punica granatum Flower Extract on Skin Injuries Induced by Burn in Rats. Adv Pharmacol Sci. 2017;2017:3059745. doi:10.1155/2017/3059745
  • 21. Eming SA, Wynn TA, Martin P. Inflammation and metabolism in tissue repair and regeneration. Science (1979). 2017;356(6342):1026-1030. doi:10.1126/SCIENCE.AAM7928,
  • 22. MacLeod AS, Mansbridge JN. The Innate Immune System in Acute and Chronic Wounds. Adv Wound Care (New Rochelle). 2016;5(2):65-78. doi:10.1089/WOUND.2014.0608,
  • 23. Putra A, Alif I, Hamra N, et al. MSC-Released TGF-? Regulate ?-SMA Expression of Myofibroblast during Wound Healing. Journal of Stem Cells & Regenerative Medicine.; 2020.
  • 24. Eming SA, Krieg T, Davidson JM. Inflammation in wound repair: Molecular and cellular mechanisms. Journal of Investigative Dermatology.Nature Publishing Group. 2007;127(3):514-525. doi:10.1038/sj.jid.5700701
  • 25. Xiao S, Huang G, Wei Z, et al. IL-10 Gene-modified human amniotic mesenchymal stem cells augment regenerative wound healing by multiple synergistic effects. Stem Cells Int. 2019;2019. doi:10.1155/2019/9158016
  • 26. Soliman AM, Barreda DR. Acute Inflammation in Tissue Healing. Int J Mol Sci. 2023;24(1). doi:10.3390/ijms24010641
  • 27. Ali F, Wajid N, Sarwar MG, Qazi AM. Oral Administration of Aloe vera Ameliorates Wound Healing through Improved Angiogenesis and chemotaxis in Sprague Dawley Rats. Curr Pharm Biotechnol. 2020;22(8):1122-1128. doi:10.2174/1389201021999201001204345,
  • 28. Arribas-López E, Zand N, Ojo O, Snowden MJ, Kochhar T. A Systematic Review of the Effect of Centella asiatica on Wound Healing. International Journal of Environmental Research and Public Health 2022, Vol 19, Page 3266. 2022;19(6):3266. doi:10.3390/IJERPH19063266
  • 29. van Loo G, Bertrand MJM. Death by TNF: a road to inflammation. Nat Rev Immunol. 2023;23(5):289-303. doi:10.1038/s41577-022-00792-3
  • 30. Lobo-Silva D, Carriche GM, Castro AG, Roque S, Saraiva M. Balancing the immune response in the brain: IL-10 and its regulation. J Neuroinflammation.BioMed Central Ltd. 2016;13(1). doi:10.1186/s12974-016-0763-8
  • 31. Arief H, Aris M, Bagian W, et al. Peranan Stres Oksidatif Pada Proses Penyembuhan Luka. Vol 5.; 2018.
  • 32. Katoh M. Fibroblast growth factor receptors as treatment targets in clinical oncology. Nat Rev Clin Oncol.Nature Publishing Group. 2019;16(2):105-122. doi:10.1038/s41571-018-0115-y
There is no Supplemental content for this article.

How to Cite This

Dewi Hartati, C., Titiek Sumarawati, & Agung Putra. (2025). Topical pomegranate (Punica granatum) extract cream accelerates wound healing by modulating VEGF and IL-10 expression in rats. Jurnal Teknologi Laboratorium, 14(2), 206–220. https://doi.org/10.29238/teknolabjournal.v14i2.516

Article Metrics

Download Statistics

Downloads

Download data is not yet available.

Other Statistics

Verify authenticity via CrossMark

Copyright and Permissions

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Publishing your paper with Jurnal Teknologi Laboratorium (JTL) means that the author or authors retain the copyright in the paper. JTL granted an exclusive reuse license by the author(s), but the author(s) are able to put the paper onto a website, distribute it to colleagues, give it to students, use it in your thesis etc, even commercially. The author(s) can reuse the figures and tables and other information contained in their paper published by JTL in future papers or work without having to ask anyone for permission, provided that the figures, tables or other information that is included in the new paper or work properly references the published paper as the source of the figures, tables or other information, and the new paper or work is not direct at private monetary gain or commercial advantage.

JTL journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. This journal is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License. This license lets others remix, transform, and build upon the material for any purpose, even commercially.

JTL journal Open Access articles are distributed under this Creative Commons Attribution-ShareAlike 4.0 International License (CC BY-SA). Articles can be read and shared for All purposes under the following conditions:

  • BY: You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
  • SA:  If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.

Data Availability

All data generated during this study are included in this published article [and its supplementary information files].