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Immunology

Hypoxia-preconditioned MSC-derived exosomes suppress IL-1beta and caspase-1 expression in a UVB-induced skin damage model

Farah Diana Sari Faculty of Medicine Biomedical Sciences Sultan Agung Islamic University Semarang, Indonesia, Indonesia Correspondence farah.diana89@yahoo.com
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Agung Putra Faculty of Medicine Biomedical Sciences Sultan Agung Islamic University Semarang, Indonesia, Indonesia Correspondence agungputra@gmail.com
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Sri Priyantini Faculty of Medicine Biomedical Sciences Sultan Agung Islamic University Semarang, Indonesia, Indonesia Correspondence sripriyantini@unissula.ac.id
Pages 70-80
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Abstract

Ultraviolet B (UVB) irradiation induces skin damage characterized by collagen degradation and enhanced inflammatory responses. Hypoxia-preconditioned exosomes derived from mesenchymal stem cells (MSCs) have emerged as a potential therapeutic strategy due to their anti-inflammatory and regenerative properties. This study aimed to evaluate the effects of hypoxia-preconditioned MSC-derived exosomes on IL-1beta and caspase-1 gene expression in a UVB-induced skin damage model. An in vivo experimental study was conducted using 30 male Wistar rats divided into three groups: control, UVB-exposed, and UVB-exposed treated with hypoxia-preconditioned MSC-derived exosomes. UVB irradiation was administered for four weeks to induce collagen degradation, followed by weekly exosome injections in the treatment group. Gene expression levels of IL-1beta and caspase-1 in skin tissue were quantified using qRT-PCR. UVB exposure significantly increased IL-1beta and caspase-1 expression compared with the control group. Treatment with hypoxia-preconditioned MSC-derived exosomes significantly reduced the expression of both inflammatory markers compared with the untreated UVB group. These findings demonstrate that hypoxia-preconditioned MSC-derived exosomes attenuate inflammasome-associated inflammatory responses and may represent a promising therapeutic approach for mitigating UVB-induced skin damage.

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How to Cite This

Sari, F. D., Agung Putra, & Sri Priyantini. (2026). Hypoxia-preconditioned MSC-derived exosomes suppress IL-1beta and caspase-1 expression in a UVB-induced skin damage model. Jurnal Teknologi Laboratorium, 15(1), 70–80. https://doi.org/10.29238/teknolabjournal.v15i1.539

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All data generated during this study are included in this published article [and its supplementary information files].