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Biotechnology

Dose-dependent modulatory effects of Pimpinella alpina (Purwoceng) root extract on TNF-α and CRP in a CCl₄-induced hepatic injury model

Viki Dwi Randa Department of Postgraduate Biomedical Science, Faculty of Medicine, Sultan Agung Islamic University, Semarang, Indonesia, Indonesia Correspondence vikidwiranda@gmail.com
,
Setyo Trisnadi Department of Postgraduate Biomedical Science, Faculty of Medicine, Sultan Agung Islamic University, Semarang, Indonesia, Indonesia Correspondence trisnadisetyo@gmail.com
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Titiek Sumarawati Department of Doctoral Biomedical Science, Faculty of Medicine, Sultan Agung Islamic University, Semarang, Indonesia, Indonesia Correspondence sumarawati@unissula.ac.id
Pages 335-345
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Abstract

The liver is highly susceptible to oxidative stress and inflammatory damage, particularly following exposure to hepatotoxic agents such as carbon tetrachloride (CCl₄). Concerns regarding the adverse effects of conventional anti-inflammatory therapies have increased interest in herbal alternatives, including purwoceng (Pimpinella alpina Molk), a medicinal plant rich in flavonoids and saponins with potential hepatoprotective activity. This study aimed to evaluate the effects of purwoceng root extract on tumor necrosis factor-alpha (TNF-alpha ) and C-reactive protein (CRP) levels in a CCl₄-induced liver injury model. Twenty-five male Wistar rats were randomly assigned to five groups: a negative control, a positive control receiving curcumin, and three treatment groups administered purwoceng extract at doses of 50, 100, or 150 mg/200 g body weight. Serum TNF-alpha and CRP levels were quantified using enzyme-linked immunosorbent assay (ELISA). Data were analyzed using the Kruskal–Wallis or one-way ANOVA tests, as appropriate. The purwoceng-treated groups exhibited lower TNF-alpha levels compared with the negative control; however, these reductions were not statistically significant. CRP levels demonstrated dose-dependent variability, with the lowest concentration observed at the 50 mg dose and a significant increase at the 150 mg dose, indicating a potential shift toward pro-oxidant or pro-inflammatory effects at higher doses. Although a statistically significant dose–response relationship was not established, these findings reflect the complex biological activity of purwoceng extract. In conclusion, purwoceng may exert modulatory effects on inflammatory markers in CCl₄-induced liver injury, but determination of an optimal therapeutic window is essential. Further studies involving bioactive compound isolation, pharmacokinetic and toxicity evaluation, and time-course analysis are warranted to clarify its hepatoprotective potential.

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How to Cite This

Randa, V. D., Trisnadi, S., & Sumarawati, T. (2025). Dose-dependent modulatory effects of Pimpinella alpina (Purwoceng) root extract on TNF-α and CRP in a CCl₄-induced hepatic injury model. Jurnal Teknologi Laboratorium, 14(2), 335–345. https://doi.org/10.29238/teknolabjournal.v14i2.574

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All data generated during this study are included in this published article [and its supplementary information files].