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Biotechnology

Modulation of Interleukin-6 and Vascular Endothelial Growth Factor by topical Noni leaf (Morinda citrifolia L.) extract cream in a rat incision wound model

Enike Putri Ananto Department of Postgraduate Biomedical Science, Faculty of Medicine, Sultan Agung Islamic University, Semarang, Indonesia, Indonesia Correspondence nikeananto@gmail.com
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Setyo Trisnadi Department of Postgraduate Biomedical Science, Faculty of Medicine, Sultan Agung Islamic University, Semarang, Indonesia, Indonesia Correspondence trisnadisetyo@gmail.com
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Danis Pertiwi Department of Clinical Pathology, Faculty of Medicine, Sultan Agung Islamic University, Semarang, Indonesia, Indonesia Correspondence danispertiwi@unissula.ac.id
Pages 299-309
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Abstract

Noni leaf (Morinda citrifolia L.) extract (NLE) has been reported to possess anti-inflammatory and pro-angiogenic properties that may support wound healing. This study evaluated the effects of topical NLE cream on interleukin-6 (IL-6) and vascular endothelial growth factor (VEGF) expression in male Wistar rats with standardized full-thickness incision wounds. Thirty rats were randomly assigned into five groups: normal control, negative control (cream base), positive control (povidone-iodine), and two treatment groups receiving 20% or 40% NLE cream. All topical treatments were applied once daily for three consecutive days. Wound tissue was collected and analyzed on day four. IL-6 levels were lowest in the normal control group (9.34 ± 6.01 pg/mL) and were significantly reduced in the 20% NLE group compared with the negative control (19.80 ± 15.95 vs. 100.8 ± 26.99 pg/mL; p = 0.002). In contrast, VEGF levels were highest in the 40% NLE group (59.76 ± 9.39 pg/mL; p < 0.01), indicating enhanced angiogenic activity. These findings suggest that topical NLE cream, particularly at higher concentrations, may promote wound healing by modulating inflammatory responses and stimulating angiogenesis, supporting its potential use as a complementary topical therapeutic agent.          

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How to Cite This

Ananto, E. P., Trisnadi, S., & Pertiwi, D. (2025). Modulation of Interleukin-6 and Vascular Endothelial Growth Factor by topical Noni leaf (Morinda citrifolia L.) extract cream in a rat incision wound model. Jurnal Teknologi Laboratorium, 14(2), 299–309. https://doi.org/10.29238/teknolabjournal.v14i2.580

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All data generated during this study are included in this published article [and its supplementary information files].