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Biotechnology

Influence of Oral Turmeric (Curcuma longa) Extract on MMP-1 Levels and Collagen Density in UV-B-Exposed Wistar Rats

Rifqoh Annisa Department of Postgraduate Biomedical Science, Faculty of Medicine, Sultan Agung Islamic University, Semarang, Indonesia, Indonesia Correspondence rifqoh.annisa1990@gmail.com
,
Pasid Harlisa Department of Postgraduate Biomedical Science, Faculty of Medicine, Sultan Agung Islamic University, Semarang, Indonesia, Indonesia Correspondence pasidharlisa@gmail.com
,
Eko Setiawan Department of Postgraduate Biomedical Science, Faculty of Medicine, Sultan Agung Islamic University, Semarang, Indonesia, Indonesia Correspondence drekosetiawan@unissula.ac.id
Pages 195-202
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Abstract

Ultraviolet-B (UV-B) radiation accelerates skin photoaging by increasing oxidative stress, matrix metalloproteinase activity, and collagen degradation. Objective: This study evaluated whether oral turmeric (Curcuma longa) extract modulates serum matrix metalloproteinase-1 (MMP-1) levels and dermal collagen density in a UV-B-exposed Wistar rat model. Twenty-four male Wistar rats were allocated into four groups (n = 6 each): normal control without UV-B exposure (G1), UV-B negative control without extract (G2), UV-B plus turmeric extract 100 mg/kgBW/day (G3), and UV-B plus turmeric extract 200 mg/kgBW/day (G4). Treatment was administered orally for 14 days. Serum MMP-1 was measured by enzyme-linked immunosorbent assay, and collagen density was assessed using Sirius Red-stained dorsal skin sections analyzed with ImageJ. The extract contained flavonoids of 24.035 mg/mL at 50 ppm and total phenols of 77.105 mg/mL at 500 ppm. MMP-1 levels were 154.38 +/- 46.34 ng/mL in G1, 197.84 +/- 20.47 ng/mL in G2, 162.45 +/- 18.13 ng/mL in G3, and 180.41 +/- 24.26 ng/mL in G4; the overall between-group comparison was not significant. Collagen density was 100.00 +/- 0.00% in G1, 71.67 +/- 2.58% in G2, 90.83 +/- 2.04% in G3, and 100.00 +/- 0.00% in G4, with significant pairwise differences between UV-B control and extract-treated groups. Oral turmeric extract improved dermal collagen density in UV-B-exposed rats, while the reduction in MMP-1 levels showed a non-significant trend. These findings support further dose-response and mechanistic studies of Curcuma longa as a photoprotective nutraceutical candidate.

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How to Cite This

Rifqoh Annisa, Pasid Harlisa, & Eko Setiawan. (2026). Influence of Oral Turmeric (Curcuma longa) Extract on MMP-1 Levels and Collagen Density in UV-B-Exposed Wistar Rats. Jurnal Teknologi Laboratorium, 15(2), 195–202. https://doi.org/10.29238/teknolabjournal.v15i2.581

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