Abstract

Maintaining hygiene through frequent hand cleaning is a fundamental practice, with hand sanitizer serving as an alternative to soap. Hand sanitizers can be synthesized using natural components such as Cassia alata L. This investigation aimed to examine the efficacy of a hand spray formulated from Cassia alata L. leaf ethanol extract in reducing the Total Plate Count (TLC) of bacteria on hands before and after application. The study employed an experimental design with a quantitative approach, utilizing a pre-test-post-test control group design involving 30 participants. Due to non-normal data distribution, the Wilcoxon Signed-Rank comparative hypothesis test was employed for data analysis. The findings revealed a statistically significant P-value of 0.001, which is less than ? (? = 0.05), indicating a substantial difference in the Total Plate Count (TLC) of bacteria on hands prior to and following the application of the Cassia alata L. leaf ethanol extract hand spray. The extract contains antimicrobial compounds like alkaloids, flavonoids, tannins, saponins and anthraquinones that can inhibit bacterial growth through various mechanisms such as disrupting cell membranes, denaturing proteins, and inactivating enzymes. Further studies are needed to enhance the composition of hand sanitizers using ethanol extract obtained from the Cassia alata L. leaves.

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References

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