Main Article Content
Malaria still become one of major health burden in Indonesia especially in remote areas of east Indonesia. Golden standard of malaria parasite detection is still microscopic technique using polychromatic light source whether from halogen or natural light source. A microscopic technique has a lot of benefits but still have weaknesses, such as time-consuming and bias on the reading by microscopist, because of artifact in the image. Aims of this study were to designed malaria parasites detection tool that is robust, fast, convenient and clear by minimizing artifact on the slide. Design of this study was laboratory experimental which modified simple microscope into an automatic microscope with table movement and webcam recording using a microcontroller and monochromatic light source. The wavelength of the light sources was 402nm(blue), 532 nm (green) and 650 nm (red), the intensity of each source differed. The reading of the slide image was conducted by two certified microscopists, who read 60 images of a thick and thin slide with three different live stage of Plasmodium falciparum live, which wearing, trophozoite and schizont. This study showed that modification of microscope was succeeded with automatic movement and webcam recording, process time in one step movement and recording approximately 10 seconds or 17minutes for 100 fields of view as confirmation process. The monochromatic light source has proven to give a clear and contrast field of view when the intensities were higher than 40 mW and the certified microscopist able to identified Plasmodium falciparum parasites. Data analysis of microscopist reading used nonparametric statistic Friedman by SPSS showed that correlation between images using monochromatic and polychromatic lights have meaningless differences in a thick and thin slide. However, hemozoin as a marker of Plasmodium falciparum parasite was less detected by monochromatic light used in this study.
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Publishing your paper with Jurnal Teknologi Laboratorium (JTL) means that the author or authors retain the copyright in the paper. JTL granted an exclusive reuse license by the author(s), but the author(s) are able to put the paper onto a website, distribute it to colleagues, give it to students, use it in your thesis etc, even commercially. The author(s) can reuse the figures and tables and other information contained in their paper published by JTL in future papers or work without having to ask anyone for permission, provided that the figures, tables or other information that is included in the new paper or work properly references the published paper as the source of the figures, tables or other information, and the new paper or work is not direct at private monetary gain or commercial advantage.
JTL journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. This journal is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License. This license lets others remix, transform, and build upon the material for any purpose, even commercially.
JTL journal Open Access articles are distributed under this Creative Commons Attribution-ShareAlike 4.0 International License (CC BY-SA). Articles can be read and shared for All purposes under the following conditions:
- BY: You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- SA: If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.
 Kemenkes RI, â€śRiset Kesehatan Dasar 2013,â€ť 2013.
 WHO, â€śInternational Travel and Health - ITH,â€ť 2012.
 J. M. BĂ©lisle et al., â€śSensitive Detection of Malaria Infection by Third Harmonic Generation Imaging,â€ť Biophys. J., vol. 94, no. 4, pp. L26â€“L28, 2008.
 â€śCONTEMPORARY,â€ť vol. 3, no. 1, 2015.
 M. Microscopy and S. Operating, â€śMicroscopy Examination of Thick and Thin Blood Films for Identification of Malaria Parasites,â€ť 2016.
 S. Lee and W. Lu, â€śUsing elastic light scattering of red blood cells to detect infection of malaria parasite,â€ť IEEE Trans. Biomed. Eng., vol. 59, no. 1, pp. 150â€“155, 2012.
 V. V. Makkapati and V. Pathangay, â€śAdaptive color illumination for microscopes,â€ť 2011 Natl. Conf. Commun. NCC 2011, pp. 1â€“5, 2011.
 P. P. Kalatiku and Y. Y. Joefrie, â€śPemrograman Motor Stepper Dengan Menggunakan Bahasa Pemrograman C,â€ť no. 20 September 2015, 2011.
 Kemenkes, Buku saku penatalaksanaan kasus malaria. 2012.
 J. I. Trisnadi, â€śSpeckle contrast reduction in laser projection displays,â€ť Proc. Soc. Photo-Optical Instrum. Eng., vol. 4657, no. 2002, pp. 131â€“137, 2002.
 E. al Zoueo, Jeremy, â€śOptical_Microscope_Based_on_Multispectral_Imaging_ .pdf.â€ť Asian network for scientific indormation, 2008.