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Clinical Chemistry

Administration of extract ethanol of Lime peel (Citrus aurantiifolia) effective as anti-hyperlipidaemia: In vivo study

Norma Farizah Fahmi Department of Medical Laboratory Technology, Noor Huda Mustofa University, Bangkalan, Indonesia, Indonesia Correspondence rezaiei.cha@gmail.com
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Dwi Aprilia Anggraini Department of Medical Laboratory Technology, Noor Huda Mustofa University, Bangkalan, Indonesia, Indonesia Correspondence dwiapriliaanggraini2021@gmail.com
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Maharani Putri Dewi Department of Medical Laboratory Technology, Noor Huda Mustofa University, Bangkalan, Indonesia, Indonesia Correspondence maharanipd24@gmail.com
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Dana Daniati Department of Midwifery, Noor Huda Mustofa University, Bangkalan, Indonesia, Indonesia Correspondence danadaniati@gmail.com
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Dian Nurmansyah Department of Medical Laboratory, Borneo Lestari University, Banjarbaru, Indonesia, Indonesia Correspondence dian.nurmansyah@unbl.ac.id
Pages 129-137
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Abstract

The quest for natural alternatives to existing pharmaceutical therapies for hyperlipidaemia has been prompted by the known harmful effects of these medications, which are a major risk factor for cardiovascular disease. Lime (Citrus aurantiifolia) peel ethanol extract was tested for its antihyperlipidemic effects on total cholesterol and triglyceride levels in mice that had been hyperlipidaemic due to a high-fat diet. In a genuine experimental study with a pre- and post-test control group design, twenty-five male Mus musculus (ddy strain), two to three months old, weighing twenty to thirty grams each, were split into five groups: one served as a negative control, another as a positive control (simvastatin 0.10 mg/20g BW), and the three treatment groups that received lime peel extract (1, 2, and 2.8 mg/20g BW) were also given a weight. The 96% ethanol maceration process yielded 1.02% of the final extract. Boiling duck egg yolk at a 20% concentration for 14 days caused hyperlipidaemia. There was confirmation of flavonoids by the Shinoda test. Enzymatic colorimetric techniques were used to evaluate lipid levels on both Day 0 and Day 7. Two-Way ANOVA and Tukey's HSD were used for data analysis (p<0.05). Flavonoids were validated by phytochemical screening. Lipid reductions were statistically substantial (p<0.001) across all treatment groups. Similar to simvastatin, the most significant decrease was shown in triglycerides (from 212.8±24.6 to 73.8±5.0 mg/dL) and cholesterol (from 275.2±24.9 to 90.6±5.4 mg/dL) at the optimum dose of 2.8 mg/20g BW. Treatment, time, and their interaction were shown to have significant impacts according to Two-Way ANOVA (p<0.001). With 2.8 mg/20g BW showing excellent antihyperlipidemic action, an ethanol extract of Citrus aurantiifolia peel successfully reduces total cholesterol and triglyceride levels in hyperlipidaemic mice, indicating its promise as a natural treatment option.

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How to Cite This

Fahmi, N. F., Anggraini , D. A., Dewi, M. P., Daniati, D., & Nurmansyah , D. (2026). Administration of extract ethanol of Lime peel (Citrus aurantiifolia) effective as anti-hyperlipidaemia: In vivo study . Jurnal Teknologi Laboratorium, 15(2), 129–137. https://doi.org/10.29238/teknolabjournal.v15i2.715

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