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Escherichia coli producing Extended-Spectrum Beta-Lactamases (ESBLs) responsible for the high number of disease and death from nosocomial infections because of an enzyme encoded by genes CTX-M. This aim of the research to identify isolates E.coli of urine patients, which is a collection of Clinical Microbiology RSUD Dr. Soetomo Surabaya on the January-February 2014. The kind of the research use is observational descriptive with molecule approachment. The sample used in clinical isolate of E.coli producing ESBLs accumulated during January-February 2014 collection Clinical Laboratory RSUD Dr. Soetomo Surabaya that is thirty isolate. The methods used for the detection of genotypic by PCR thus electrophoresis and visualized on agarose gel 1.5%. The results show that twenty-seven isolate (90%) positive containing a gen CTX-M with the highest number found on the Interna Departement. Detection Bacteria producing ESBLs in genotype important that therapy an antibiotic the patients are given more effective and efficient.
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