Kadar Protein Total dan Ureum Dengan dan Tanpa Penambahan γ-cyclodextrin Pada Serum Lipemik

Main Article Content

Sujono Sujono Yumna Ayu Maulida Meilanda Puspita Sari

Abstract

Lipemic serum is a turbidity serum caused by accumulation of lipoprotein particle. This case distrupt any kinds of examination at clinic laboratory which one of them is examining Total Protein. It can be carried out by adding γ-cyclodextrin in order to handle the turbidity on lipemic serum. This study aims at finding the Total Protein’s levels overview on lipemic serum with and without addition of γ-cyclodextrin. This study was pre-eksperimental design byusing Post Test Only with Control Group Design. This study was done at Chemistry Laboratory, Health Analysis Study Program in April 2016. The sample of the data was 20 lipemic serum. In examining of total proteis’s level used Biuret method and ureum level used Barthelot method from lipemic serum with and without addition ofγ-cyclodextrin 20%.There is a significant difference between total protein and ureum, the difference average of total protein level and ureum level before and after adding γ-cyclodextrin was 1,311 g/dL (12,40%) and 6.38 mg/dL (10.88%).

Downloads

Download data is not yet available.

Article Details

How to Cite
Sujono, S., Maulida, Y., & Sari, M. (2016). Kadar Protein Total dan Ureum Dengan dan Tanpa Penambahan γ-cyclodextrin Pada Serum Lipemik. Jurnal Teknologi Laboratorium, 5(1), 16 -19. Retrieved from https://teknolabjournal.com/index.php/Jtl/article/view/71
Section
Clinical Chemistry

References

[1] Sacher, R.A. dan McPherson, R.A. 2004. Tinjauan Klinis Hasil Pemeriksaan Laboratorium. Alih Bahasa: Brahm U.P. dan Dewi Wulandari. Jakarta: EGC.
[2] Masruroh, A. 2014. Korelasi antara Kadar Trigliserida dengan Kadar Kolesterol pada Serum Lipemik. Karya Tulis Ilmiah. Surabaya: Perpustakaan UM Surabaya.
[3] Piyophirapong, S.M.D., Wongtiraporn, W.M.D., Sribhen, K.M.D. 2010. Factitious Result in Clinical Chemstry Tests Caused by Common Endogenous Interferents. Siriraj Medical Journal, volume 62 Number 4, July-August 2010.
[4] Diasys. 2008. Manual for Glucose. Germany: Diagnostic Systems
[5] Sharma, A., Anderson, K., dan Baker, J.W. 1990. Flocculation of Serum Lipoprotein with Cyclodextrin : Application to Assay of Hyperlipidemic Serum. Clinica Chemistry.Journal Clinical Chemistry Vol. 36, No. 3.Central Michigan University.
[6] Nikolac, N. 2013. Lipemia: Causes, Interference Mechanisms, Detection and Management. Biochemia Medica 2014;24(1):57-67. Kroasia: University Departement of Chemistry.
[7] Roberts C.M. dan Cotten S.W. 2013. Cyclodextrin Removal of Lipemic Interference: An Attractive Alternative to Ultracentrifugation for Satellite Laboratories. Arch Pathol Lab Med—Vol 137, August 2013
[8] Castro, A.R., Morrill, W.E., dan Pope, V. 2000. Lipid Removal from Human Serum Sample. AST Laboratory Research Journal, Mar.2000, p.197-199 Vol.7, No.2. Atlanta: Clinical and Diagnostic Laboratory Immunology.
[9] Itsnaini, Y.Z., 2015.Penggunaan Flokulan Alfa-Siklodekstrin Pada Serum Lipemik Terhadap Pemeriksaan Kadar Protein Total. Karya TulisIlmiah.Yogkyakarta: Jurusan Analis Kesehatan Poltekkes KemenkesYogyakarta.
[10] Robert, C.M. and Cotton, S.W. 2013. Cyclodexttrin Removal of Lipemic Interference: An attractive Alternative to Ultracentrifugation for Satellite Laboratories. Archive of Pathology and Laboratory Medicine: August 2013, Vol 137 (8), 1027-1028.
[11] Sugiyono. 2012. Metode Penelitian Kuantitatif, Kualitatif, dan Kombinasi (Mixed Methods). Bandung: Alfabeta.
[12] Sharma, A., Anderson, K. and Baker, J.B. 1990. Flocculation of Serum Lipoproteins with Cyclodextrins: Application to Assay of Hyperlipemic Serum. Journal of Clinical Chemistry,Vol. 36 (3),529-532.