Kajian Aktivitas Antikanker Ekstrak Daun Gude (cajanus cajan) Terhadap Sel Kanker Kolon Secara in Vitro

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Muji Rahayu Roosmarinto Roosmarinto

Abstract

Colorectal cancer is one of the five type of highest insiden in Indonesia. One of development cancer therapeutics directed to combination of chemotherapeutic agent and chemopreventive compounds. One approach to finding a chemopreventive compounds is through the exploration of natural ingredient especialy herbs. Flavonoids is group of compounds from plants that have been widely studied as anti-cancer activity. Leave of pigeonpea (Cajanus cajan) is, kind of Legumes plants rich in flavonoids among others cajanol, quercetin, luteolin, apigenin formonentin, vitexin, orientin, biochanin A, pinostrobin, dan isorhamnetin. This study aims to determine the content of chemical compounds in the methanol extract of leave pigeon pea that has anticancer activity using WiDr colorectal cancer cells with MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) method, followed by flowcytometry test. The result of the cytotoxicity test of methanol extract of pigeonpea leaves have IC50 of 307 ug/ml and with flowcytometry method showed necrosis WiDr cell.

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How to Cite
Rahayu, M., & Roosmarinto, R. (2017). Kajian Aktivitas Antikanker Ekstrak Daun Gude (cajanus cajan) Terhadap Sel Kanker Kolon Secara in Vitro. Jurnal Teknologi Laboratorium, 6(1), 31 - 38. https://doi.org/https://doi.org/10.29238/teknolabjournal.v6i1.87
Section
Food and Drink Chemistry

References

[1] C. Kanadaswami, L. T. Lee, P. P. H. Lee, J. J. Hwang, F. C. Ke, Y. T. Huang, and M. T. Lee, “The antitumor activities of flavonoids,” In Vivo (Brooklyn)., vol. 19, no. 5, pp. 895–910, 2005.
[2] J. Ferlay, I. Soerjomataram, R. Dikshit, S. Eser, C. Mathers, M. Rebelo, D. M. Parkin, D. Forman, and F. Bray, “Cancer incidence and mortality worldwide : Sources , methods and major patterns in GLOBOCAN 2012,” Int. J. Cancer, vol. 386, no. 136, pp. E358–E386, 2015.
[3] N. Loganayaki, P. Siddhuraju, and S. Manian, “A comparative study on in vitro antioxidant activity of the legumes Acacia auriculiformis and Acacia ferruginea with a conventional legume Cajanus cajan Estudio comparativo de la actividad antioxidante in vitro de las legumbres Acacia auriculiformis y Aca,” CyTA J. Food, vol. 9 No. 1, no. December 2014, pp. 37–41, 2011.
[4] A. Nix, C. A. Paull, and M. Colgrave, “The flavonoid profile of pigeonpea, Cajanus cajan: a review,” Springerplus, vol. 4, no. 1, p. 125, 2015.
[5] Aja, P.M., Alum, E.U., Ezeani, N. and N. Nwali, B.U., Edwin, “Comparative Phytochemical Composition of Cajanus cajan Leaf and Seed,” Intl. J. Microbiol. Res., vol. 6, no. 1, pp. 42–46, 2015.
[6] J. Y. Chahar MK, Sharma N, Dobhal MP, “Flavonoids: A versatile source of anticancer drugs.,” Pharmacogn. Rev., vol. 5, no. 9, pp. 1–12, 2011.
[7] S. Ramos, “Review Cancer chemoprevention and chemotherapy : Dietary polyphenols and signalling pathways,” Mol Nutr Food Res, vol. 52, pp. 507–526, 2008.[8] D. I. Hertzog and O. S. Tica, “Review Molecular mechanisms underlying the anti- cancerous action of flavonoids,” Curr. Heal. Sci. J., vol. 38, no. 4, pp. 145–149, 2012.
[9] S. Lim, J. Xu, J. Kim, T. Chen, X. Su, J. Standard, and W. Wang, “Role of Anthocyanin-enriched Purple-fleshed Sweet Pottao P40 in Colorectal Cancer Prevention,” Mol Nutr Food Res, vol. 57, no. 11, pp. 1908–1917, 2013.
[10] International Agency for Research in Cancer, 2012. GLOBOCAN: Estimated Cancer Incidence, Mortality and Prevalence Worldwide in 2012, WHO.